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基本情報
登録情報 | データベース: PDB / ID: 1rrp | ||||||
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タイトル | STRUCTURE OF THE RAN-GPPNHP-RANBD1 COMPLEX | ||||||
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![]() | COMPLEX (SMALL GTPASE/NUCLEAR PROTEIN) / COMPLEX (SMALL GTPASE-NUCLEAR PROTEIN) / SMALL GTPASE / NUCLEAR TRANSPORT / COMPLEX (SMALL GTPASE-NUCLEAR PROTEIN) complex | ||||||
機能・相同性 | ![]() cytoplasmic periphery of the nuclear pore complex / SUMO ligase complex / SUMO ligase activity / annulate lamellae / RNA nuclear export complex / pre-miRNA export from nucleus / nuclear pore cytoplasmic filaments / snRNA import into nucleus / cellular response to mineralocorticoid stimulus / manchette ...cytoplasmic periphery of the nuclear pore complex / SUMO ligase complex / SUMO ligase activity / annulate lamellae / RNA nuclear export complex / pre-miRNA export from nucleus / nuclear pore cytoplasmic filaments / snRNA import into nucleus / cellular response to mineralocorticoid stimulus / manchette / Nuclear Pore Complex (NPC) Disassembly / nuclear inclusion body / nuclear pore nuclear basket / Transport of Ribonucleoproteins into the Host Nucleus / Regulation of Glucokinase by Glucokinase Regulatory Protein / Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) / Regulation of cholesterol biosynthesis by SREBP (SREBF) / Transport of the SLBP independent Mature mRNA / importin-alpha family protein binding / Transport of the SLBP Dependant Mature mRNA / NS1 Mediated Effects on Host Pathways / SUMOylation of SUMOylation proteins / Transport of Mature mRNA Derived from an Intronless Transcript / protein localization to nucleolus / Rev-mediated nuclear export of HIV RNA / SUMOylation of RNA binding proteins / Nuclear import of Rev protein / nuclear export / 転移酵素; アシル基を移すもの; アミノアシル基を移すもの / GTP metabolic process / NEP/NS2 Interacts with the Cellular Export Machinery / Transport of Mature mRNA derived from an Intron-Containing Transcript / tRNA processing in the nucleus / SUMO transferase activity / Postmitotic nuclear pore complex (NPC) reformation / nucleocytoplasmic transport / MicroRNA (miRNA) biogenesis / centrosome localization / Viral Messenger RNA Synthesis / DNA metabolic process / dynein intermediate chain binding / regulation of gluconeogenesis / NLS-bearing protein import into nucleus / SUMOylation of ubiquitinylation proteins / Vpr-mediated nuclear import of PICs / mitotic sister chromatid segregation / SUMOylation of DNA replication proteins / protein sumoylation / spermatid development / ribosomal large subunit export from nucleus / Regulation of HSF1-mediated heat shock response / sperm flagellum / mRNA transport / ribosomal subunit export from nucleus / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / SUMOylation of DNA damage response and repair proteins / nuclear pore / ribosomal small subunit export from nucleus / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Resolution of Sister Chromatid Cohesion / response to amphetamine / centriole / viral process / protein export from nucleus / SUMOylation of chromatin organization proteins / mitotic spindle organization / G protein activity / HCMV Late Events / male germ cell nucleus / hippocampus development / RHO GTPases Activate Formins / Transcriptional regulation by small RNAs / 加水分解酵素; 酸無水物に作用; GTPに作用・細胞または細胞小器官の運動に関与 / recycling endosome / ISG15 antiviral mechanism / small GTPase binding / HCMV Early Events / positive regulation of protein import into nucleus / protein import into nucleus / Separation of Sister Chromatids / GDP binding / Signaling by ALK fusions and activated point mutants / melanosome / protein folding / positive regulation of protein binding / nuclear envelope / mitotic cell cycle / snRNP Assembly / midbody / actin cytoskeleton organization / nuclear membrane / cadherin binding / protein heterodimerization activity / protein domain specific binding / cell division / intracellular membrane-bounded organelle / GTPase activity / chromatin binding / protein-containing complex binding 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Vetter, I.R. / Nowak, C. / Nishimoto, T. / Kuhlmann, J. / Wittinghofer, A. | ||||||
![]() | ![]() タイトル: Structure of a Ran-binding domain complexed with Ran bound to a GTP analogue: implications for nuclear transport. 著者: Vetter, I.R. / Nowak, C. / Nishimoto, T. / Kuhlmann, J. / Wittinghofer, A. #1: ![]() タイトル: Ranbp1 is Crucial for the Release of Rangtp from Importin Beta-Related Nuclear Transport Factors 著者: Bischoff, F.R. / Gorlich, D. #2: ![]() タイトル: Dynamic and Equilibrium Studies on the Interaction of Ran with its Effector, Ranbp1 著者: Kuhlmann, J. / Macara, I. / Wittinghofer, A. #3: ![]() タイトル: A Ran-Binding Motif Found in Nuclear Pore Proteins 著者: Hartmann, E. / Gorlich, D. #4: ![]() タイトル: A Giant Nucleopore Protein that Binds Ran/Tc4 著者: Yokoyama, N. / Hayashi, N. / Seki, T. / Pante, N. / Ohba, T. / Nishii, K. / Kuma, K. / Hayashida, T. / Miyata, T. / Aebi, U. / Fukui, M. / Nishimoto, T. #5: ![]() タイトル: The C Terminus of the Nuclear Ran/Tc4 Gtpase Stabilizes the Gdp-Bound State and Mediates Interactions with Rcc1, Ran-Gap, and Htf9A/Ranbp1 著者: Richards, S.A. / Lounsbury, K.M. / Macara, I.G. #6: ![]() タイトル: Co-Activation of Rangtpase and Inhibition of GTP Dissociation by Ran-GTP Binding Protein Ranbp1 著者: Bischoff, F.R. / Krebber, H. / Smirnova, E. / Dong, W. / Ponstingl, H. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 147.1 KB | 表示 | ![]() |
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PDB形式 | ![]() | 115.1 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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非結晶学的対称性 (NCS) | NCS oper: (Code: given Matrix: (0.9936, -0.046, 0.1032), ベクター: |
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要素
#1: タンパク質 | 分子量: 23183.807 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() #2: タンパク質 | 分子量: 15703.035 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() #3: 化合物 | #4: 化合物 | #5: 水 | ChemComp-HOH / | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 3.6 Å3/Da / 溶媒含有率: 60 % | ||||||||||||||||||||
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結晶化 | pH: 6.25 / 詳細: pH 6.25 | ||||||||||||||||||||
結晶 | *PLUS | ||||||||||||||||||||
結晶化 | *PLUS 手法: unknown | ||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: SIEMENS / 検出器: AREA DETECTOR / 日付: 1998年4月1日 |
放射 | 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.84 Å / 相対比: 1 |
反射 | 解像度: 2.9→20 Å / Num. obs: 20286 / % possible obs: 86 % / Observed criterion σ(I): 2 / 冗長度: 4.5 % / Biso Wilson estimate: 75.4 Å2 / Rmerge(I) obs: 0.091 / Net I/σ(I): 13.1 |
反射 シェル | 解像度: 2.9→3 Å / Rmerge(I) obs: 0.191 / Mean I/σ(I) obs: 3.1 / % possible all: 66 |
反射 | *PLUS Num. measured all: 90705 |
反射 シェル | *PLUS % possible obs: 66 % |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: RAN-GDP 解像度: 2.96→12.5 Å / Rfactor Rfree error: 0.007 / Isotropic thermal model: RESTRAINED / 交差検証法: THROUGHOUT / σ(F): 2
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溶媒の処理 | 溶媒モデル: FLAT MODEL / Bsol: 38.4 Å2 / ksol: 0.24 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 56.1 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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精密化ステップ | サイクル: LAST / 解像度: 2.96→12.5 Å
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拘束条件 |
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Refine LS restraints NCS | NCS model details: CONSTR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Xplor file |
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ソフトウェア | *PLUS 名称: CNS / バージョン: 0.4 / 分類: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
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