+
Open data
-
Basic information
Entry | Database: PDB / ID: 1rq7 | ||||||
---|---|---|---|---|---|---|---|
Title | MYCOBACTERIUM TUBERCULOSIS FTSZ IN COMPLEX WITH GDP | ||||||
![]() | Cell division protein ftsZ | ||||||
![]() | CELL CYCLE / SIGNALING PROTEIN / TUBULIN / GTPASE | ||||||
Function / homology | ![]() septin ring assembly / chloroplast fission / FtsZ-dependent cytokinesis / division septum assembly / cell division site / protein polymerization / positive regulation of cell cycle / cell division / GTPase activity / GTP binding ...septin ring assembly / chloroplast fission / FtsZ-dependent cytokinesis / division septum assembly / cell division site / protein polymerization / positive regulation of cell cycle / cell division / GTPase activity / GTP binding / magnesium ion binding / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Leung, A.K.W. / White, E.L. / Ross, L.J. / Reynolds, R.C. / DeVito, J.A. / Borhani, D.W. | ||||||
![]() | ![]() Title: Structure of Mycobacterium tuberculosis FtsZ reveals unexpected, G protein-like conformational switches. Authors: Leung, A.K. / Lucile White, E. / Ross, L.J. / Reynolds, R.C. / DeVito, J.A. / Borhani, D.W. #1: ![]() Title: Polymerization of C-Terminally Truncated Mycobacterium tuberculosis FtsZ Is Unlikely to be Physiologically Relevant Authors: Borhani, D.W. / White, E.L. #2: Journal: Acta Crystallogr.,Sect.D / Year: 2000 Title: Crystallization of the Mycobacterium tuberculosis cell-division protein FtsZ Authors: Leung, A.K.W. / White, E.L. / Ross, L.J. / Borhani, D.W. #3: Journal: J.Bacteriol. / Year: 2000 Title: Slow polymerization of Mycobacterium tuberculosis FtsZ Authors: White, E.L. / Ross, L.J. / Reynolds, R.C. / Seitz, L.E. / Moore, G.D. / Borhani, D.W. #4: Journal: J.ANTIMICROB.CHEMOTHER. / Year: 2002 Title: 2-Alkoxycarbonylaminopyridines: inhibitors of Mycobacterium tuberculosis FtsZ Authors: White, E.L. / Suling, W.J. / Ross, L.J. / Seitz, L.E. / Reynolds, R.C. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 124.8 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 94.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 800.1 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 806.1 KB | Display | |
Data in XML | ![]() | 24.2 KB | Display | |
Data in CIF | ![]() | 34.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1rluC ![]() 1rq2SC C: citing same article ( S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 39073.004 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-GDP / | #3: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.58 Å3/Da / Density % sol: 53 % Description: GDP INTRODUCED BY SOAKING CRYSTAL, GROWN UNDER FTSZ/CITRATE COMPLEX CONDITIONS (PDB ENTRY 1RQ2), WITH 2MM GDP AND 10MM MGCL2 OVERNIGHT. |
---|---|
Crystal grow | Temperature: 293 K / pH: 5.6 Details: 30% PEG 4000, 0.1M SODIUM CITRATE, 0.2M AMMONIUM ACETATE, 2MM SRI-7614, ETHYL (6-AMINO-2,3-DIHYDRO-4-PHENYL-1H-PYRIDO[4,3-B][1,4]DIAZEPIN-8-YL)-CARBAMATE, WAS INCLUDED AS WELL, BUT WAS NOT ...Details: 30% PEG 4000, 0.1M SODIUM CITRATE, 0.2M AMMONIUM ACETATE, 2MM SRI-7614, ETHYL (6-AMINO-2,3-DIHYDRO-4-PHENYL-1H-PYRIDO[4,3-B][1,4]DIAZEPIN-8-YL)-CARBAMATE, WAS INCLUDED AS WELL, BUT WAS NOT LOCATED IN THE FINAL STRUCTURE, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 5.60 |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jun 29, 2000 / Details: DUAL OSMIC MULTILAYER MIRRORS |
Radiation | Monochromator: DUAL OSMIC MULTILAYER MIRRORS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→15 Å / Num. obs: 23644 / % possible obs: 96.6 % / Observed criterion σ(I): 0 / Redundancy: 2.6 % / Biso Wilson estimate: 29 Å2 / Rmerge(I) obs: 0.119 / Rsym value: 0.119 / Net I/σ(I): 6.7 |
Reflection shell | Resolution: 2.6→2.69 Å / Redundancy: 1.8 % / Rmerge(I) obs: 0.476 / Mean I/σ(I) obs: 1.7 / Rsym value: 0.476 / % possible all: 77.3 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PARTIALLY REFINED MODEL OF THE MYCOBACTERIUM TUBERCULOSIS FTSZ/CITRATE COMPLEX, PDB ENTRY 1RQ2. Resolution: 2.6→14.99 Å / Cor.coef. Fo:Fc: 0.939 / Cor.coef. Fo:Fc free: 0.897 / SU B: 10.206 / SU ML: 0.214 / Isotropic thermal model: isotropic / Cross valid method: THROUGHOUT / ESU R: 0.475 / ESU R Free: 0.287 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: X-PLOR-GENERATED BULK SOLVENT PARTIAL STRUCTURE FACTORS WERE USED IN REFMAC AS PARTIAL STRUCTURE FACTORS (FPART/PHIPART), EXCEPT IN LAST ROUND, WHERE REFMAC BABINET MODEL WITH MASK WAS USED.
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 38.108 Å2
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→14.99 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.6→2.666 Å / Total num. of bins used: 20 /
|