+Open data
-Basic information
Entry | Database: PDB / ID: 1rlu | ||||||
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Title | Mycobacterium tuberculosis FtsZ in complex with GTP-gamma-S | ||||||
Components | Cell division protein ftsZ | ||||||
Keywords | CELL CYCLE / SIGNALING PROTEIN / TUBULIN / GTPASE | ||||||
Function / homology | Function and homology information septin ring assembly / FtsZ-dependent cytokinesis / division septum assembly / cell division site / protein polymerization / positive regulation of cell cycle / cell division / GTPase activity / GTP binding / magnesium ion binding ...septin ring assembly / FtsZ-dependent cytokinesis / division septum assembly / cell division site / protein polymerization / positive regulation of cell cycle / cell division / GTPase activity / GTP binding / magnesium ion binding / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Mycobacterium tuberculosis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.08 Å | ||||||
Authors | Leung, A.K.W. / White, E.L. / Ross, L.J. / Reynolds, R.C. / DeVito, J.A. / Borhani, D.W. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2004 Title: Structure of Mycobacterium tuberculosis FtsZ reveals unexpected, G protein-like conformational switches. Authors: Leung, A.K. / Lucile White, E. / Ross, L.J. / Reynolds, R.C. / DeVito, J.A. / Borhani, D.W. #1: Journal: To be Published Title: Polymerization of C-Terminally Truncated Mycobacterium tuberculosis FtsZ Is Unlikely to be Physiologically Relevant Authors: Borhani, D.W. / White, E.L. #2: Journal: Acta Crystallogr.,Sect.D / Year: 2000 Title: Crystallization of the Mycobacterium tuberculosis cell-division protein FtsZ Authors: Leung, A.K.W. / White, E.L. / Ross, L.J. / Borhani, D.W. #3: Journal: J.Bacteriol. / Year: 2000 Title: Slow polymerization of Mycobacterium tuberculosis FtsZ Authors: White, E.L. / Ross, L.J. / Reynolds, R.C. / Seitz, L.E. / Moore, G.D. / Borhani, D.W. #4: Journal: J.ANTIMICROB.CHEMOTHER. / Year: 2002 Title: 2-Alkoxycarbonylaminopyridines: inhibitors of Mycobacterium tuberculosis FtsZ Authors: White, E.L. / Suling, W.J. / Ross, L.J. / Seitz, L.E. / Reynolds, R.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1rlu.cif.gz | 129 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1rlu.ent.gz | 97.5 KB | Display | PDB format |
PDBx/mmJSON format | 1rlu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rl/1rlu ftp://data.pdbj.org/pub/pdb/validation_reports/rl/1rlu | HTTPS FTP |
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-Related structure data
Related structure data | 1rq2SC 1rq7C S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 39073.004 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mycobacterium tuberculosis (bacteria) / Gene: FTSZ, RV2150C, MT2209, MTCY270.18, MB2174C / Plasmid: pJD168 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)-pLysS / References: UniProt: P64170, UniProt: P9WN95*PLUS #2: Chemical | ChemComp-GSP / | #3: Chemical | ChemComp-GOL / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.58 Å3/Da / Density % sol: 53 % Description: GTP-GAMMA-S INTRODUCED BY SOAKING CRYSTAL, GROWN UNDER FTSZ/CITRATE COMPLEX CONDITIONS (PDB ENTRY 1RQ2), WITH 2MM GTP -GAMMA-S AND 10MM MGCL2 OVERNIGHT. |
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Crystal grow | Temperature: 293 K / pH: 5.6 Details: 30% PEG 4000, 0.1M sodium citrate, 0.2M ammonium acetate, 2MM SRI-7614, ETHYL (6-AMINO-2,3-DIHYDRO-4-PHENYL-1H-PYRIDO[4,3-B][1,4]DIAZEPIN-8-YL)-CARBAMATE, WAS INCLUDED AS WELL, BUT WAS NOT ...Details: 30% PEG 4000, 0.1M sodium citrate, 0.2M ammonium acetate, 2MM SRI-7614, ETHYL (6-AMINO-2,3-DIHYDRO-4-PHENYL-1H-PYRIDO[4,3-B][1,4]DIAZEPIN-8-YL)-CARBAMATE, WAS INCLUDED AS WELL, BUT WAS NOT LOCATED IN THE FINAL STRUCTURE, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 5.60 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: CHESS / Beamline: A1 / Wavelength: 0.9235 |
Detector | Type: ADSC QUANTUM 1 / Detector: CCD / Date: Jul 29, 2000 / Details: MIRRORS/MONOCHROMATOR |
Radiation | Monochromator: SI 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9235 Å / Relative weight: 1 |
Reflection | Resolution: 2.08→15 Å / Num. obs: 44782 / % possible obs: 94.6 % / Observed criterion σ(I): 0 / Redundancy: 4.3 % / Biso Wilson estimate: 29.9 Å2 / Rsym value: 0.089 / Net I/σ(I): 9.7 |
Reflection shell | Resolution: 2.08→2.15 Å / Redundancy: 1.7 % / Mean I/σ(I) obs: 1.7 / Rsym value: 0.439 / % possible all: 50.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PARTIALLY REFINED MODEL OF THE MYCOBACTERIUM TUBERCULOSIS FTSZ/CITRATE COMPLEX, PDB ENTRY 1RQ2. Resolution: 2.08→14.94 Å / Cor.coef. Fo:Fc: 0.959 / Cor.coef. Fo:Fc free: 0.94 / SU B: 4.281 / SU ML: 0.113 / Cross valid method: THROUGHOUT / ESU R: 0.168 / ESU R Free: 0.158 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: X-PLOR-GENERATED BULK SOLVENT PARTIAL STRUCTURE FACTORS WERE USED IN REFMAC AS PARTIAL STRUCTURE FACTORS (FPART/PHIPART), EXCEPT IN LAST ROUND, WHERE REFMAC BABINET MODEL WITH MASK WAS USED.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 36.924 Å2
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Refinement step | Cycle: LAST / Resolution: 2.08→14.94 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.08→2.133 Å / Total num. of bins used: 20 /
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