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Yorodumi- PDB-1rq6: Solution structure of ribosomal protein S17E from Methanobacteriu... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1rq6 | ||||||
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Title | Solution structure of ribosomal protein S17E from Methanobacterium Thermoautotrophicum, Northeast Structural Genomics Consortium Target TT802 / Ontario Center for Structural Proteomics Target Mth0803 | ||||||
Components | 30S ribosomal protein S17eRibosome | ||||||
Keywords | TRANSLATION / alpha protein / Structural Genomics / Protein Structure Initiative / PSI / NESG / Northeast Structural Genomics Consortium | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Methanothermobacter thermautotrophicus (archaea) | ||||||
Method | SOLUTION NMR / torsion angle dyanmics | ||||||
Authors | Wu, B. / Yee, A. / Huang, Y.J. / Ramelot, T.A. / Semesi, A. / Jung, J.W. / Edward, A. / Lee, W. / Kennedy, M.A. / Montelione, G.T. ...Wu, B. / Yee, A. / Huang, Y.J. / Ramelot, T.A. / Semesi, A. / Jung, J.W. / Edward, A. / Lee, W. / Kennedy, M.A. / Montelione, G.T. / Arrowsmith, C.H. / Northeast Structural Genomics Consortium (NESG) | ||||||
Citation | Journal: Protein Sci. / Year: 2008 Title: The solution structure of ribosomal protein S17E from Methanobacterium thermoautotrophicum: a structural homolog of the FF domain. Authors: Wu, B. / Yee, A. / Huang, Y.J. / Ramelot, T.A. / Cort, J.R. / Semesi, A. / Jung, J.W. / Lee, W. / Montelione, G.T. / Kennedy, M.A. / Arrowsmith, C.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1rq6.cif.gz | 300.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1rq6.ent.gz | 261.1 KB | Display | PDB format |
PDBx/mmJSON format | 1rq6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rq/1rq6 ftp://data.pdbj.org/pub/pdb/validation_reports/rq/1rq6 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 7213.341 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Methanothermobacter thermautotrophicus (archaea) Gene: RPS17E, MTH803 / Plasmid: PET15B / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: O26894 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1 mM mth0803, U-15N,13C; 450 mM NaCl, 25 mM Na2PO4, 1mM Benzamidine, 1xinhibitor cooktail, 0.01% NaN3, 95% H2O, 5% D2O Solvent system: 95% H2O/5% D2O |
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Sample conditions | Ionic strength: 450 mM NaCl, 25 mM Phosphate buffer / pH: 6.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: torsion angle dyanmics / Software ordinal: 1 Details: 1143 NOE-derived distance constraints and 159 angle constraints (PHI and PSI) | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations,target function Conformers calculated total number: 112 / Conformers submitted total number: 15 |