Mass: 19202.123 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: LOCATED IN THE LARGE 50S RIBOSOMAL SUBUNIT BELOW THE L7/L12 STALK AND CLOSE TO THE GTPASE CENTER Source: (gene. exp.) Geobacillus stearothermophilus (bacteria) Cellular location: RIBOSOME / Plasmid: PET-13A / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P02391
Mass: 18.015 Da / Num. of mol.: 140 / Source method: isolated from a natural source / Formula: H2O
Compound details
THE PROTEIN CONTAINS TWO DOMAINS OF APPROXIMATELY EQUAL SIZE. THE DOMAINS HAVE VERY SIMILAR ALPHA ...THE PROTEIN CONTAINS TWO DOMAINS OF APPROXIMATELY EQUAL SIZE. THE DOMAINS HAVE VERY SIMILAR ALPHA BETA STRUCTURES AND SECONDARY STRUCTURE TOPOLOGIES AND APPEAR TO HAVE ARISEN BY GENE DUPLICATION. EACH DOMAIN IS ALSO HOMOLOGOUS TO THE RNA RECOGNITION MOTIF (RRM), WITH THE C-TERMINAL DOMAIN BEING THE MOST SIMILAR.
-
Experimental details
-
Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
-
Sample preparation
Crystal
Density Matthews: 2.4 Å3/Da / Density % sol: 50 % Description: MUTANT V124C WAS CONSTRUCTED FOR THE MERCURY DERIVATIVE
Crystal grow
pH: 7.6 / Details: pH 7.6
Components of the solutions
ID
Name
Crystal-ID
Sol-ID
1
NA/KPHOSPHATE
1
1
2
BETAMERCAPTOETHANOL
1
1
3
1,4-DIOXANE
1
1
Crystal grow
*PLUS
Method: vapor diffusion, hanging drop / PH range low: 7.8 / PH range high: 7.2
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