[English] 日本語
Yorodumi- PDB-1rfb: CRYSTAL STRUCTURE OF RECOMBINANT BOVINE INTERFERON-GAMMA AT 3.0 A... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1rfb | ||||||
---|---|---|---|---|---|---|---|
Title | CRYSTAL STRUCTURE OF RECOMBINANT BOVINE INTERFERON-GAMMA AT 3.0 ANGSTROMS RESOLUTION | ||||||
Components | INTERFERON-GAMMA | ||||||
Keywords | GLYCOPROTEIN | ||||||
Function / homology | Function and homology information Interferon gamma signaling / Regulation of IFNG signaling / IFNG signaling activates MAPKs / type II interferon receptor binding / humoral immune response / positive regulation of B cell proliferation / cytokine activity / defense response to virus / adaptive immune response / positive regulation of transcription by RNA polymerase II / extracellular space Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 3 Å | ||||||
Authors | Samudzi, C.T. / Rubin, J.R. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 1993 Title: Structure of recombinant bovine interferon-gamma at 3.0 A resolution. Authors: Samudzi, C.T. / Rubin, J.R. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1rfb.cif.gz | 60 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1rfb.ent.gz | 46 KB | Display | PDB format |
PDBx/mmJSON format | 1rfb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1rfb_validation.pdf.gz | 376.6 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1rfb_full_validation.pdf.gz | 428.6 KB | Display | |
Data in XML | 1rfb_validation.xml.gz | 13.5 KB | Display | |
Data in CIF | 1rfb_validation.cif.gz | 18.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rf/1rfb ftp://data.pdbj.org/pub/pdb/validation_reports/rf/1rfb | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Atom site foot note | 1: SER B 19 - PRO B 20 OMEGA =279.70 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION 2: GLY B 26 - PRO B 27 OMEGA =290.90 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION |
-Components
#1: Protein | Mass: 14055.080 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bos taurus (cattle) / References: UniProt: P07353 #2: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
---|
-Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 52.63 % | |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | *PLUS Temperature: 17 ℃ / pH: 6 / Method: vapor diffusion | |||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Radiation | Scattering type: x-ray |
---|---|
Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.66 Å / Num. obs: 7891 / Num. measured all: 19800 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Rfactor Rwork: 0.192 / Rfactor obs: 0.192 / Highest resolution: 3 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 3 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 3 Å / Lowest resolution: 8 Å / Num. reflection obs: 2744 / σ(I): 2 / Rfactor obs: 0.192 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
|