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Yorodumi- PDB-1re0: Structure of ARF1-GDP bound to Sec7 domain complexed with Brefeldin A -
+Open data
-Basic information
Entry | Database: PDB / ID: 1re0 | ||||||
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Title | Structure of ARF1-GDP bound to Sec7 domain complexed with Brefeldin A | ||||||
Components |
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Keywords | PROTEIN TRANSPORT / all-helical / alph-beta | ||||||
Function / homology | Function and homology information guanyl-nucleotide exchange factor activity => GO:0005085 / Synthesis of PIPs at the Golgi membrane / synaptic vesicle budding / lysosomal membrane organization / positive regulation of late endosome to lysosome transport / regulation of phospholipid metabolic process / Synthesis of PIPs at the plasma membrane / Golgi to transport vesicle transport / phospholipase D activator activity / Intra-Golgi traffic ...guanyl-nucleotide exchange factor activity => GO:0005085 / Synthesis of PIPs at the Golgi membrane / synaptic vesicle budding / lysosomal membrane organization / positive regulation of late endosome to lysosome transport / regulation of phospholipid metabolic process / Synthesis of PIPs at the plasma membrane / Golgi to transport vesicle transport / phospholipase D activator activity / Intra-Golgi traffic / COPI-dependent Golgi-to-ER retrograde traffic / COPI-mediated anterograde transport / cellular bud / Golgi cis cisterna / trans-Golgi Network Vesicle Budding / mitotic cleavage furrow ingression / trans-Golgi Network Vesicle Budding / positive regulation of ER to Golgi vesicle-mediated transport / regulation of ARF protein signal transduction / Lysosome Vesicle Biogenesis / regulation of receptor internalization / very-low-density lipoprotein particle assembly / regulation of Arp2/3 complex-mediated actin nucleation / Golgi Associated Vesicle Biogenesis / Intra-Golgi traffic / MHC class II antigen presentation / positive regulation of calcium ion-dependent exocytosis / postsynaptic actin cytoskeleton organization / Synthesis of PIPs at the Golgi membrane / intra-Golgi vesicle-mediated transport / retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum / Nef Mediated CD4 Down-regulation / dendritic spine organization / long-term synaptic depression / COPI-dependent Golgi-to-ER retrograde traffic / positive regulation of dendritic spine development / Lysosome Vesicle Biogenesis / extrinsic component of membrane / positive regulation of sodium ion transmembrane transport / Golgi Associated Vesicle Biogenesis / positive regulation of endocytosis / cell leading edge / Synthesis of PIPs at the plasma membrane / intracellular copper ion homeostasis / endoplasmic reticulum to Golgi vesicle-mediated transport / endomembrane system / COPI-mediated anterograde transport / vesicle-mediated transport / MHC class II antigen presentation / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / sarcomere / small monomeric GTPase / actin filament organization / positive regulation of protein secretion / macroautophagy / intracellular protein transport / cellular response to virus / GDP binding / late endosome / actin cytoskeleton organization / postsynaptic density / neuron projection / protein domain specific binding / Golgi membrane / focal adhesion / GTPase activity / glutamatergic synapse / GTP binding / perinuclear region of cytoplasm / Golgi apparatus / magnesium ion binding / endoplasmic reticulum / protein-containing complex / mitochondrion / RNA binding / extracellular exosome / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | ||||||
Authors | Goldberg, J. / Mossessova, E. | ||||||
Citation | Journal: Mol.Cell / Year: 2003 Title: Crystal structure of ARF1*Sec7 complexed with Brefeldin A and its implications for the guanine nucleotide exchange mechanism. Authors: Mossessova, E. / Corpina, R.A. / Goldberg, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1re0.cif.gz | 91.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1re0.ent.gz | 67.3 KB | Display | PDB format |
PDBx/mmJSON format | 1re0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/re/1re0 ftp://data.pdbj.org/pub/pdb/validation_reports/re/1re0 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 2 types, 2 molecules AB
#1: Protein | Mass: 18822.455 Da / Num. of mol.: 1 / Fragment: Truncated form of ARF1 (residues 17-180) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ARF1 / Plasmid: pET / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)pLysS / References: UniProt: P32889, UniProt: P84077*PLUS |
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#2: Protein | Mass: 25418.918 Da / Num. of mol.: 1 / Fragment: Core Sec7 domain of Gea1 (residues 540-754) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: Gea1 / Production host: Escherichia coli (E. coli) / References: UniProt: P47102 |
-Non-polymers , 5 types, 117 molecules
#3: Chemical | ChemComp-MG / |
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#4: Chemical | ChemComp-GDP / |
#5: Chemical | ChemComp-AFB / |
#6: Chemical | ChemComp-CIT / |
#7: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.16 Å3/Da / Density % sol: 61.12 % | |||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: PEG 400, sodium citrate, Tris-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K | |||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X25 / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD |
Radiation | Monochromator: Si 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→20 Å / Num. all: 22531 / Num. obs: 22531 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Biso Wilson estimate: 37.5 Å2 / Rmerge(I) obs: 0.061 |
Reflection shell | Resolution: 2.4→2.49 Å / % possible all: 99.9 |
Reflection | *PLUS Num. measured all: 136824 |
Reflection shell | *PLUS % possible obs: 99.9 % / Num. unique obs: 2221 / Num. measured obs: 13339 / Rmerge(I) obs: 0.249 / Mean I/σ(I) obs: 6.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.4→19.95 Å / Rfactor Rfree error: 0.007 / Data cutoff high absF: 1201695.34 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 30.7622 Å2 / ksol: 0.348137 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso mean: 42.6 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.4→19.95 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.4→2.55 Å / Rfactor Rfree error: 0.022 / Total num. of bins used: 6
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Xplor file |
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Refinement | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 20 Å / % reflection Rfree: 5 % / Rfactor Rfree: 0.238 / Rfactor Rwork: 0.211 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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