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- PDB-1r9k: Representative solution structure of the catalytic domain of SopE2 -
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Open data
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Basic information
Entry | Database: PDB / ID: 1r9k | ||||||
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Title | Representative solution structure of the catalytic domain of SopE2 | ||||||
![]() | TypeIII-secreted protein effector: invasion-associated protein | ||||||
![]() | CELL INVASION / Salmonella / invasion / type III / GEF / SopE | ||||||
Function / homology | ![]() GTPase activator activity / guanyl-nucleotide exchange factor activity / actin cytoskeleton organization / extracellular region Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Williams, C. / Galyov, E.E. / Bagby, S. | ||||||
![]() | ![]() Title: Solution Structure, Backbone Dynamics, and Interaction with Cdc42 of Salmonella Guanine Nucleotide Exchange Factor SopE2(,). Authors: Williams, C. / Galyov, E.E. / Bagby, S. #1: ![]() Title: Biochemical and structural analysis of Salmonella and Burkholderia virulence proteins Authors: Williams, C. #2: ![]() Title: Assignment of the 1H,13C and 15N resonances of the catalytic domain of guanine nucelotide exchange factor SopE2 from Salmonella dublin Authors: Williams, C. / Galyov, E.E. / Bagby, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 67.6 KB | Display | ![]() |
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PDB format | ![]() | 51.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 347 KB | Display | ![]() |
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Full document | ![]() | 369.8 KB | Display | |
Data in XML | ![]() | 8.2 KB | Display | |
Data in CIF | ![]() | 10.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1r6eC C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 18585.338 Da / Num. of mol.: 1 / Fragment: SopE2 GEF domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy. |
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Sample preparation
Details |
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Sample conditions |
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-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: he structures are based on a total of 3065 restraints, 2682 are NOE-derived distance constraints, 249 dihedral angle restraints,134 distance restraints from hydrogen bonds. | ||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with acceptable covalent geometry,structures with the least restraint violations,structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 1 |