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Open data
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Basic information
Entry | Database: PDB / ID: 1r2z | ||||||
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Title | MutM (Fpg) bound to 5,6-dihydrouracil (DHU) containing DNA | ||||||
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![]() | HYDROLASE/DNA / DNA REPAIR / DNA GLYCOSYLASE / HYDROLASE-DNA COMPLEX | ||||||
Function / homology | ![]() DNA-formamidopyrimidine glycosylase / 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / base-excision repair / damaged DNA binding / zinc ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fromme, J.C. / Verdine, G.L. | ||||||
![]() | ![]() Title: DNA Lesion Recognition by the Bacterial Repair Enzyme MutM. Authors: Fromme, J.C. / Verdine, G.L. | ||||||
History |
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Remark 999 | SEQUENCE The sequence of this protein is not available in any reference sequence database. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 89.5 KB | Display | ![]() |
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PDB format | ![]() | 63.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 448.7 KB | Display | ![]() |
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Full document | ![]() | 451.2 KB | Display | |
Data in XML | ![]() | 16.5 KB | Display | |
Data in CIF | ![]() | 24.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1r2yC ![]() 1l1tS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: DNA chain | Mass: 3687.417 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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#2: DNA chain | Mass: 3599.340 Da / Num. of mol.: 1 / Source method: obtained synthetically |
#3: Protein | Mass: 30738.525 Da / Num. of mol.: 1 / Mutation: E3Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: mutm / Plasmid: pET24b / Production host: ![]() ![]() |
#4: Chemical | ChemComp-ZN / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.8 Å3/Da / Density % sol: 56.05 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: PEG 8000, MAGNESIUM ACETATE, SODIUM CACODYLATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Method: vapor diffusion / Details: Fromme, J.C., (2002) Nat.Struct.Biol., 9, 544. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jun 30, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 1.63→50 Å / Num. all: 54565 / Num. obs: 53944 / % possible obs: 98.9 % / Observed criterion σ(I): -3 / Redundancy: 5.3 % / Biso Wilson estimate: 19.3 Å2 / Rmerge(I) obs: 0.048 / Net I/σ(I): 31 |
Reflection shell | Resolution: 1.63→1.69 Å / Redundancy: 4.8 % / Rmerge(I) obs: 0.357 / Mean I/σ(I) obs: 4 / Num. unique all: 5369 / % possible all: 93.9 |
Reflection | *PLUS Lowest resolution: 50 Å |
Reflection shell | *PLUS % possible obs: 93.9 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 1L1T Resolution: 1.63→50 Å / Rfactor Rfree error: 0.005 / Data cutoff high absF: 314184.8 / Data cutoff high rms absF: 314184.8 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 49.7263 Å2 / ksol: 0.372355 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 29.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.63→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.63→1.69 Å / Rfactor Rfree error: 0.016 / Total num. of bins used: 10
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Xplor file |
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Refinement | *PLUS Lowest resolution: 50 Å | ||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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