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Open data
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Basic information
Entry | Database: PDB / ID: 1qos | |||||||||
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Title | lectin UEA-II complexed with chitobiose | |||||||||
![]() | CHITIN BINDING LECTIN, UEA-II | |||||||||
![]() | LECTIN / CARBOHYDRATE BINDING / N-ACETYLGLUCOSAMINE | |||||||||
Function / homology | ![]() | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Loris, R. / De Greve, H. / Dao-Thi, M.-H. / Messens, J. / Imberty, A. / Wyns, L. | |||||||||
![]() | ![]() Title: Structural Basis of Carbohydrate Recognition by Lectin II from Ulex Europaeus, a Protein with a Promiscuous Carbohydrate Binding Site Authors: Loris, R. / De Greve, H. / Dao-Thi, M.-H. / Messens, J. / Imberty, A. / Wyns, L. #1: Journal: Curr.Opin.Struct.Biol. / Year: 1999 Title: Novel Structures of Plant Lectins and Their Complexes with Carbohydrates Authors: Bouckaert, J. / Hamelryck, T.W. / Wyns, L. / Loris, R. / Hamelryck, T.W. #2: Journal: Acta Crystallogr.,Sect.D / Year: 1998 Title: The Quaternary Structure of Uea-II, the Chitobiose Specific Lectin from Gorse Authors: Dao-Thi, M.-H. / Rizkallah, P. / Wyns, L. / Poortmans, F. / Loris, R. #3: Journal: Biochim.Biophys.Acta / Year: 1998 Title: Legume Lectin Structure Authors: Loris, R. / Hamelryck, T.W. / Bouckaert, J. / Wyns, L. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 104.4 KB | Display | ![]() |
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PDB format | ![]() | 79.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 18.9 KB | Display | |
Data in CIF | ![]() | 24.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1dzqC ![]() 1qnwC ![]() 1qooSC ![]() 1qotC C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26093.008 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #2: Polysaccharide | Source method: isolated from a genetically manipulated source #3: Chemical | #4: Chemical | #5: Sugar | ChemComp-NAG / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.71 Å3/Da / Density % sol: 54.54 % | ||||||||||||||||||||||||
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Crystal grow | pH: 6.5 / Details: pH 6.50 | ||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 45 % | ||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging dropDetails: Dao-Thi M.H., (1998) Acta Crystallog. sect., D54, 844. | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 287 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Details: MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.909 Å / Relative weight: 1 |
Reflection | Resolution: 2.95→20 Å / Num. obs: 12117 / % possible obs: 98.2 % / Observed criterion σ(I): -3 / Redundancy: 3.91 % / Rmerge(I) obs: 0.135 / Net I/σ(I): 10.18 |
Reflection shell | Resolution: 2.95→3.08 Å / Redundancy: 4.02 % / Rmerge(I) obs: 0.55 / Mean I/σ(I) obs: 3.24 / % possible all: 99.1 |
Reflection | *PLUS Num. measured all: 47378 |
Reflection shell | *PLUS % possible obs: 99.1 % / Num. unique obs: 1200 / Num. measured obs: 4824 / Rmerge(I) obs: 0.55 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: UEA-II COMPLEXED WITH NAG (1QOO) Resolution: 2.95→20 Å / Data cutoff high absF: 0 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.95→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.75→3.08 Å / Total num. of bins used: 8
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.352 / Rfactor obs: 0.29 |