THE ASYMMETRIC UNIT CONTAINS AN INSULIN DIMER. THREE DIMERS AGGREGATE AROUND TWO ZINC IONS SITUATED ON A CRYSTALLOGRAPHIC3-FOLD AXIS TO FORM A HEXAMER.A MEAN DIFFERENCE IN ACCESSIBLE SURFACE AREA PER THE CHAIN IN THE COMPLEX OF 1325.5 ANGSTROM**2
pH: 6.8 詳細: CRYSTALLISATION IN BATCH: 10 MG B5 TYR INSULIN DISSOLVED IN 2 ML 0.02M HCL. TO THIS ADDED 0.05 ML 0.15M ZINC ACETATE, 1.0 ML 0.2 M TRI-SODIUM CITRATE, 1.0 ML ACETONE. PH ADJUSTED TO 6.4-7.1 .
結晶化
*PLUS
手法: batch method / PH range low: 7.1 / PH range high: 6.4
溶液の組成
*PLUS
ID
濃度
一般名
Crystal-ID
Sol-ID
化学式
詳細
1
10mg/ml
insulin
1
1
2
0.02M
1
1
HCl
2ml
3
0.15M
zincacetate
1
1
0.05ml
4
0.2M
trisodiumcitrate
1
1
1ml
5
acetone
1
1
1ml
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データ収集
回折
平均測定温度: 289 K
放射光源
由来: 回転陽極 / タイプ: RIGAKU RUH2R / 波長: 1.5418
検出器
タイプ: RIGAKU IMAGE PLATE / 検出器: IMAGE PLATE
放射
モノクロメーター: GRAPHITE(002) / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.5418 Å / 相対比: 1
反射
解像度: 2.4→25.7 Å / Num. obs: 3477 / % possible obs: 97 % / 冗長度: 2.5 % / Biso Wilson estimate: 30.9 Å2 / Rmerge(I) obs: 0.046
反射 シェル
解像度: 2→2.1 Å / 冗長度: 2.3 % / Rmerge(I) obs: 0.132 / % possible all: 92
反射 シェル
*PLUS
% possible obs: 92 %
-
解析
ソフトウェア
名称
分類
PROLSQ
精密化
MOSFLM
データ削減
SCALA
データスケーリング
精密化
構造決定の手法: 分子置換 開始モデル: T3R3 (NATIVE) INSULIN 解像度: 2.4→25.7 Å / σ(F): 0 詳細: THE FOLLOWING SIDECHAINS HAVE BEEN ASSIGNED ZERO OCCUPANCIES DUE TO DISORDER: A4, A5, A10, B3, B4, B21, B29, C14, D3, D4 THE FOLLOWING CHAIN TERMINAL RESIDUES HAVE BEEN ASSIGNED ZERO ...詳細: THE FOLLOWING SIDECHAINS HAVE BEEN ASSIGNED ZERO OCCUPANCIES DUE TO DISORDER: A4, A5, A10, B3, B4, B21, B29, C14, D3, D4 THE FOLLOWING CHAIN TERMINAL RESIDUES HAVE BEEN ASSIGNED ZERO OCCUPANCIES DUE TO DISORDER: B1-B2, B30, D30 THE FOLLOWING A CHAIN RESIDUES HAVE BEEN ASSIGNED ZERO OR HALF OCCUPANCIES DUE TO DISORDER, RESULTING FROM THE B5 HIS TO TYR MUTATION: A7, A8, A9