+Open data
-Basic information
Entry | Database: PDB / ID: 1qhn | ||||||
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Title | CHLORAMPHENICOL PHOSPHOTRANSFERASE FROM STREPTOMYCES VENEZUELAE | ||||||
Components | CHLORAMPHENICOL PHOSPHOTRANSFERASE | ||||||
Keywords | TRANSFERASE / KINASE / ANTIBIOTIC RESISTANCE / PHOSPHORYLATION / MONONUCLEOTIDE BINDING FOLD | ||||||
Function / homology | Function and homology information Transferases; Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor / kinase activity / phosphorylation / response to antibiotic / ATP binding Similarity search - Function | ||||||
Biological species | Streptomyces venezuelae (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.7 Å | ||||||
Authors | Izard, T. | ||||||
Citation | Journal: Embo J. / Year: 2000 Title: The Crystal Structures of Chloramphenicol Phosphotransferase Reveal a Novel Inactivation Mechanism Authors: Izard, T. / Ellis, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1qhn.cif.gz | 46.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1qhn.ent.gz | 34.3 KB | Display | PDB format |
PDBx/mmJSON format | 1qhn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qh/1qhn ftp://data.pdbj.org/pub/pdb/validation_reports/qh/1qhn | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 19115.734 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Streptomyces venezuelae (bacteria) / Strain: ISP5230 References: UniProt: Q56148, Transferases; Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor |
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#2: Chemical | ChemComp-SO4 / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 8.8 Å3/Da / Density % sol: 86 % | |||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.5 / Details: pH 7.50 | |||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion | |||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: BM14 / Wavelength: 0.9785, 0.9783, 0.88 | ||||||||||||
Detector | Detector: CCD | ||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.7→20 Å / Num. obs: 18936 / % possible obs: 99.5 % / Redundancy: 13.52 % / Biso Wilson estimate: 54.9 Å2 / Rmerge(I) obs: 0.128 / Rsym value: 12.8 / Net I/σ(I): 11.1 | ||||||||||||
Reflection shell | Resolution: 2.7→2.78 Å / Rmerge(I) obs: 0.368 / Rsym value: 36.8 / % possible all: 100 | ||||||||||||
Reflection | *PLUS Num. measured all: 255940 | ||||||||||||
Reflection shell | *PLUS % possible obs: 100 % |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 2.7→99 Å / Rfactor Rfree error: 0.007 / Data cutoff high absF: 9801121.83 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 35.1 Å2 / ksol: 0.344 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 32.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.7→99 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.7→2.87 Å / Rfactor Rfree error: 0.022 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.5 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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