[English] 日本語
![](img/lk-miru.gif)
- PDB-1qex: BACTERIOPHAGE T4 GENE PRODUCT 9 (GP9), THE TRIGGER OF TAIL CONTRA... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1qex | ||||||
---|---|---|---|---|---|---|---|
Title | BACTERIOPHAGE T4 GENE PRODUCT 9 (GP9), THE TRIGGER OF TAIL CONTRACTION AND THE LONG TAIL FIBERS CONNECTOR | ||||||
![]() | PROTEIN (BACTERIOPHAGE T4 GENE PRODUCT 9 (GP9)) | ||||||
![]() | VIRAL PROTEIN / BACTERIOPHAGE T4 / BASEPLATE / GENE PRODUCT 9 / OLIGOMERIZATION | ||||||
Function / homology | ![]() virus tail, baseplate / viral tail assembly / viral release from host cell Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Kostyuchenko, V.A. / Navruzbekov, G.A. / Kurochkina, L.P. / Strelkov, S.V. / Mesyanzhinov, V.V. / Rossmann, M.G. | ||||||
![]() | ![]() Title: The structure of bacteriophage T4 gene product 9: the trigger for tail contraction. Authors: Kostyuchenko, V.A. / Navruzbekov, G.A. / Kurochkina, L.P. / Strelkov, S.V. / Mesyanzhinov, V.V. / Rossmann, M.G. #1: Journal: J.Mol.Biol. / Year: 1993 Title: Crystallization and preliminary crystallographic characterization of bacteriophage T4 baseplate protein encoded by gene 9. Authors: Strelkov, S.V. / Zurabishvili, T.G. / Nepluev, I.V. / Efimov, V.P. / Isupov, M.N. / Harutyunyan, E.H. / Mesyanzhinov, V.V. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 125.5 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 98.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 451.6 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 471.7 KB | Display | |
Data in XML | ![]() | 28 KB | Display | |
Data in CIF | ![]() | 38.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
Unit cell |
| ||||||||
Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.49937, 0.86639, 0.00032), Vector: |
-
Components
#1: Protein | Mass: 31024.725 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 3.1 Å3/Da / Density % sol: 60 % | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | pH: 7.5 / Details: 28% PEG-400 0.2M CACL2 HEPES-NA BUFFER, PH 7.5 | ||||||||||||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 110 K |
---|---|
Diffraction source | Source: ![]() |
Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Jun 1, 1997 / Details: MIRRORS |
Radiation | Monochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→40 Å / Num. obs: 33400 / % possible obs: 97.5 % / Observed criterion σ(I): -2 / Redundancy: 5.8 % / Biso Wilson estimate: 31.7 Å2 / Rmerge(I) obs: 0.071 / Net I/σ(I): 15 |
Reflection shell | Resolution: 2.3→2.4 Å / Redundancy: 5.6 % / Rmerge(I) obs: 0.17 / Mean I/σ(I) obs: 5 / % possible all: 96.3 |
Reflection shell | *PLUS % possible obs: 96.3 % |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Details: DISORDERED REGIONS WERE MODELLED STEREOCHEMICALLY AND MARKED BY HIGH B-FACTORS
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Solvent model: FLAT MODEL / Bsol: 36.78 Å2 / ksol: 0.33 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 34.5 Å2
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→20 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.3→2.4 Å / Rfactor Rfree error: 0.02 / Total num. of bins used: 8
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Xplor file |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: CNS / Version: 0.5 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 20 Å / σ(F): 2 / % reflection Rfree: 4.9 % / Rfactor obs: 0.23 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 34.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | *PLUS Highest resolution: 2.3 Å / Rfactor Rfree: 0.304 / % reflection Rfree: 5.5 % / Rfactor Rwork: 0.28 |