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Open data
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Basic information
Entry | Database: PDB / ID: 1qbm | ||||||
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Title | FAB E8B ANTIBODY, X-RAY STRUCTURE AT 2.37 ANGSTROMS RESOLUTION | ||||||
![]() | (E8B ANTIBODY) x 2 | ||||||
![]() | IMMUNOGLOBULIN / IGG1 KAPPA / ANTIBODY / FAB FRAGMENT / ANTI-CYTOCHROME C ANTIBODY | ||||||
Function / homology | ![]() immunoglobulin complex / adaptive immune response / immune response / extracellular space Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Mylvaganam, S.E. / Paterson, Y. / Getzoff, E.D. | ||||||
![]() | ![]() Title: Structural basis for the binding of an anti-cytochrome c antibody to its antigen: crystal structures of FabE8-cytochrome c complex to 1.8 A resolution and FabE8 to 2.26 A resolution. Authors: Mylvaganam, S.E. / Paterson, Y. / Getzoff, E.D. #1: ![]() Title: Biochemical Implications from the Variable Gene Sequences of an Anti-Cytochrome C Antibody and Crystallographic Characterization of its Antigen-Binding Fragment in Free and Antigen-Complexed Forms Authors: Mylvaganam, S.E. / Paterson, Y. / Kaiser, K. / Bowdish, K. / Getzoff, E.D. #2: ![]() Title: Monoclonal Antibodies to Horse Cytochrome C Expressing Four Distinct Idiotypes Distribute Among Two Sites on the Native Protein Authors: Carbone, F.R. / Paterson, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 98.1 KB | Display | ![]() |
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PDB format | ![]() | 74.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 426.9 KB | Display | ![]() |
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Full document | ![]() | 438.8 KB | Display | |
Data in XML | ![]() | 19.4 KB | Display | |
Data in CIF | ![]() | 27 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Antibody | Mass: 23655.057 Da / Num. of mol.: 1 / Fragment: FAB FRAGMENT / Source method: isolated from a natural source / Details: E8 ANTIBODY PURIFIED FROM ASCITES / Source: (natural) ![]() ![]() |
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#2: Antibody | Mass: 23505.213 Da / Num. of mol.: 1 / Fragment: FAB FRAGMENT / Source method: isolated from a natural source / Details: E8 ANTIBODY PURIFIED FROM ASCITES / Source: (natural) ![]() ![]() |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 43 % | |||||||||||||||
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Crystal grow | pH: 6.4 / Details: pH 6.4 | |||||||||||||||
Crystal grow | *PLUS pH: 6.2 / Method: unknown | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 291 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: SIEMENS / Detector: AREA DETECTOR |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Highest resolution: 2.37 Å / Num. obs: 17924 / % possible obs: 91.6 % / Redundancy: 3 % / Biso Wilson estimate: 45.2 Å2 / Rsym value: 0.146 / Net I/σ(I): 14 |
Reflection shell | Resolution: 2.37→2.47 Å / Mean I/σ(I) obs: 2 / Rsym value: 0.44 / % possible all: 52.3 |
Reflection | *PLUS Rmerge(I) obs: 0.146 |
Reflection shell | *PLUS % possible obs: 52.3 % / Rmerge(I) obs: 0.44 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2.26 ANGSTROM RESOLUTION FAB E8A Resolution: 2.37→10 Å / σ(F): 0
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Displacement parameters | Biso mean: 36.6 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.37→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.37→2.48 Å / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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