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- PDB-1q2e: CELLOBIOHYDROLASE CEL7A WITH LOOP DELETION 245-252 AND BOUND NON-... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1q2e | ||||||||||||
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Title | CELLOBIOHYDROLASE CEL7A WITH LOOP DELETION 245-252 AND BOUND NON-HYDROLYSABLE CELLOTETRAOSE | ||||||||||||
![]() | EXOCELLOBIOHYDROLASE I | ||||||||||||
![]() | HYDROLASE / CELLULASE / CELLULOSE DEGRADATION / GLYCOSIDASE / GLYCOPROTEIN / GLYCOSYLATED PROTEIN / LOOP DELETION / CELLOTETRAOSE | ||||||||||||
Function / homology | ![]() cellulose 1,4-beta-cellobiosidase (non-reducing end) / cellulose 1,4-beta-cellobiosidase activity / cellulose binding / cellulose catabolic process / extracellular region Similarity search - Function | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | ![]() ![]() | ||||||||||||
![]() | Stahlberg, J. / Harris, M. / Jones, T.A. | ||||||||||||
![]() | ![]() Title: Engineering the exo-loop of Trichoderma reesei cellobiohydrolase, Cel7A. A comparison with Phanerochaete chrysosporium Cel7D Authors: von Ossowski, I. / Stahlberg, J. / Koivula, A. / Piens, K. / Becker, D. / Boer, H. / Harle, R. / Harris, M. / Divne, C. / Mahdi, S. / Zhao, Y. / Driguez, H. / Claeyssens, M. / Sinnott, M.L. / Teeri, T.T. #1: ![]() Title: Activity Studies and Crystal Structures of Catalytically Deficient Mutants of Cellobiohydrolase I from Trichoderma Reesei Authors: Stahlberg, J. / Divne, C. / Koivula, A. / Piens, K. / Claeyssens, M. / Teeri, T.T. / Jones, T.A. #2: ![]() Title: High-Resolution Crystal Structures Reveal How a Cellulose Chain is Bound in the 50A Long Tunnel of Cellobiohydrolase-I from Trichoderma Reesei Authors: Divne, C. / Stahlberg, J. / Teeri, T.T. / Jones, T.A. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 178.3 KB | Display | ![]() |
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PDB format | ![]() | 143.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 953.7 KB | Display | ![]() |
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Full document | ![]() | 958.4 KB | Display | |
Data in XML | ![]() | 37.3 KB | Display | |
Data in CIF | ![]() | 55.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 45110.777 Da / Num. of mol.: 2 / Fragment: CATALYTIC DOMAIN 1-434 / Mutation: 245-252 DELETION Source method: isolated from a genetically manipulated source Details: COMPLEXED TO METHYL 4-S-BETA-CELLOBIOSYL-4-THIO-BETA-CELLOBIOSIDE Source: (gene. exp.) ![]() ![]() References: UniProt: P00725, UniProt: P62694*PLUS, cellulose 1,4-beta-cellobiosidase (non-reducing end) #2: Polysaccharide | Type: oligosaccharide / Mass: 696.669 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source #3: Sugar | #4: Chemical | ChemComp-CA / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.97 Å3/Da / Density % sol: 37 % | |||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7 Details: PEG 5000, TRIS-HCL, ETHYLENE GLYCOL, CALCIUM CHLORIDE, SODIUM ACETATE, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K | |||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 5 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Sep 21, 1999 / Details: mirrors |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→40 Å / Num. obs: 78914 / % possible obs: 100 % / Observed criterion σ(I): 0 / Redundancy: 4.1 % / Biso Wilson estimate: 12.6 Å2 / Rmerge(I) obs: 0.046 / Net I/σ(I): 21.3 |
Reflection shell | Resolution: 1.75→1.81 Å / Redundancy: 3.8 % / Rmerge(I) obs: 0.132 / % possible all: 99.9 |
Reflection | *PLUS Highest resolution: 1.75 Å / Lowest resolution: 40 Å / Redundancy: 4.1 % |
Reflection shell | *PLUS % possible obs: 99.9 % / Redundancy: 3.8 % |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 1.75→20 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 40 Å / Num. reflection obs: 76597 / % reflection Rfree: 2 % | |||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||
Displacement parameters | *PLUS |