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Open data
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Basic information
Entry | Database: PDB / ID: 1psr | ||||||
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Title | HUMAN PSORIASIN (S100A7) | ||||||
![]() | PSORIASIN | ||||||
![]() | EF-HAND PROTEIN / MAD PHASING / PSORIASIS / S100 PROTEIN FAMILY | ||||||
Function / homology | ![]() positive regulation of granulocyte chemotaxis / zinc ion sequestering activity / Metal sequestration by antimicrobial proteins / positive regulation of T cell chemotaxis / RAGE receptor binding / positive regulation of monocyte chemotaxis / epidermis development / keratinocyte differentiation / response to reactive oxygen species / calcium-dependent protein binding ...positive regulation of granulocyte chemotaxis / zinc ion sequestering activity / Metal sequestration by antimicrobial proteins / positive regulation of T cell chemotaxis / RAGE receptor binding / positive regulation of monocyte chemotaxis / epidermis development / keratinocyte differentiation / response to reactive oxygen species / calcium-dependent protein binding / azurophil granule lumen / antimicrobial humoral immune response mediated by antimicrobial peptide / angiogenesis / collagen-containing extracellular matrix / response to lipopolysaccharide / positive regulation of ERK1 and ERK2 cascade / focal adhesion / calcium ion binding / Neutrophil degranulation / endoplasmic reticulum / extracellular space / zinc ion binding / extracellular region / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Brodersen, D.E. / Etzerodt, M. / Madsen, P. / Celis, J. / Thoegersen, H.C. / Nyborg, J. / Kjeldgaard, M. | ||||||
![]() | ![]() Title: EF-hands at atomic resolution: the structure of human psoriasin (S100A7) solved by MAD phasing. Authors: Brodersen, D.E. / Etzerodt, M. / Madsen, P. / Celis, J.E. / Thogersen, H.C. / Nyborg, J. / Kjeldgaard, M. #1: ![]() Title: Crystallization and Preliminary X-Ray Diffraction Studies of Psoriasin Authors: Nolsoe, S. / Thirup, S. / Etzerodt, M. / Thoegersen, H.C. / Nyborg, J. #2: ![]() Title: Molecular Cloning, Occurrence, and Expression of a Novel Partially Secreted Protein "Psoriasin" that is Highly Up-Regulated in Psoriatic Skin Authors: Madsen, P. / Rasmussen, H.H. / Leffers, H. / Honore, B. / Dejgaard, K. / Olsen, E. / Kiil, J. / Walbum, E. / Andersen, A.H. / Basse, B. / Lauridsen, J.B. / Ratz, G.P. / Celis, A. / ...Authors: Madsen, P. / Rasmussen, H.H. / Leffers, H. / Honore, B. / Dejgaard, K. / Olsen, E. / Kiil, J. / Walbum, E. / Andersen, A.H. / Basse, B. / Lauridsen, J.B. / Ratz, G.P. / Celis, A. / Vandekerckhove, J. / Celis, J.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 108.6 KB | Display | ![]() |
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PDB format | ![]() | 89 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.7741, 0.413, -0.4797), Vector: |
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Components
#1: Protein | Mass: 11343.784 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: CA2+ SUBSTITUTED FOR HO3+ / Source: (gene. exp.) ![]() Cellular location: CYTOPLASMIC OR MAY BE SECRETED BY A NON-CLASSICAL SECRETORY PATHWAY Plasmid: PT7H6FX-PS.4 / Species (production host): Escherichia coli / Production host: ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 39 % | |||||||||||||||||||||||||
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Crystal grow | pH: 5.5 / Details: pH 5.5 | |||||||||||||||||||||||||
Crystal | *PLUS | |||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, sitting drop / PH range low: 7.5 / PH range high: 4.6 | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR scanner 300 mm plate / Detector: IMAGE PLATE / Date: Apr 1, 1997 / Details: SEGMENTED MIRROR |
Radiation | Monochromator: BENT SINGLE-CRYSTAL GERMANIUM TRIANGULAR MONOCHROMATOR Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9091 Å / Relative weight: 1 |
Reflection | Resolution: 1.05→37.5 Å / Num. obs: 160437 / % possible obs: 97.9 % / Redundancy: 1.8 % / Rsym value: 0.033 / Net I/σ(I): 28.7 |
Reflection shell | Resolution: 1.05→1.07 Å / Redundancy: 1.18 % / Mean I/σ(I) obs: 4.7 / Rsym value: 0.103 / % possible all: 84.1 |
Reflection | *PLUS Num. obs: 84591 / Num. measured all: 700858 / Rmerge(I) obs: 0.043 |
Reflection shell | *PLUS % possible obs: 84.1 % / Rmerge(I) obs: 0.101 |
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Processing
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Refinement | Method to determine structure: MULTI-WAVELENGTH ANOMALOUS DISPERSION Highest resolution: 1.05 Å / Num. parameters: 18753 / Num. restraintsaints: 19286 / Cross valid method: FREE R / σ(F): 0 / Stereochemistry target values: ENGH AND HUBER
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Solvent computation | Solvent model: MOEWS & KRETSINGER, J.MOL.BIOL. (1973) 91: 201-228 | |||||||||||||||||||||||||||||||||
Refine analyze | Num. disordered residues: 19 / Occupancy sum hydrogen: 1394.2 / Occupancy sum non hydrogen: 1925.6 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 1.05 Å
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Refine LS restraints |
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Software | *PLUS Name: SHELXL-97 / Classification: refinement | |||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 37.5 Å / Num. reflection obs: 160437 / Rfactor obs: 0.1049 / Rfactor Rwork: 0.1072 | |||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |