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Yorodumi- PDB-1phe: CRYSTAL STRUCTURES OF METYRAPONE-AND PHENYLIMIDAZOLE-INHIBITED CO... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1phe | ||||||
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Title | CRYSTAL STRUCTURES OF METYRAPONE-AND PHENYLIMIDAZOLE-INHIBITED COMPLEXES OF CYTOCHROME P450-CAM | ||||||
Components | CYTOCHROME P450-CAM | ||||||
Keywords | OXIDOREDUCTASE(OXYGENASE) | ||||||
Function / homology | Function and homology information camphor 5-monooxygenase / camphor 5-monooxygenase activity / (+)-camphor catabolic process / cholest-4-en-3-one 26-monooxygenase activity / steroid hydroxylase activity / cholesterol catabolic process / iron ion binding / heme binding / cytoplasm Similarity search - Function | ||||||
Biological species | Pseudomonas putida (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.6 Å | ||||||
Authors | Poulos, T.L. | ||||||
Citation | Journal: Biochemistry / Year: 1987 Title: Crystal structures of metyrapone- and phenylimidazole-inhibited complexes of cytochrome P-450cam. Authors: Poulos, T.L. / Howard, A.J. #1: Journal: Biochemistry / Year: 1993 Title: Inhibitor-Induced Conformational Change in Cytochrome P450-Cam Authors: Raag, R. / Li, H. / Jones, B.C. / Poulos, T.L. #2: Journal: Biochemistry / Year: 1986 Title: Crystal Structure of Substrate-Free Pseudomonas Putida Cytochrome P450 Authors: Poulos, T.L. / Finzel, B.C. / Howard, A.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1phe.cif.gz | 97.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1phe.ent.gz | 74.8 KB | Display | PDB format |
PDBx/mmJSON format | 1phe.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1phe_validation.pdf.gz | 494.8 KB | Display | wwPDB validaton report |
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Full document | 1phe_full_validation.pdf.gz | 510.1 KB | Display | |
Data in XML | 1phe_validation.xml.gz | 11.9 KB | Display | |
Data in CIF | 1phe_validation.cif.gz | 18.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ph/1phe ftp://data.pdbj.org/pub/pdb/validation_reports/ph/1phe | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUE 89 IS A CIS PROLINE. / 2: RESIDUE 100 IS A CIS PROLINE. / 3: RESIDUE 106 IS A CIS PROLINE. / 4: SEE REMARK 8. |
-Components
#1: Protein | Mass: 46588.879 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas putida (bacteria) / References: UniProt: P00183, camphor 5-monooxygenase |
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#2: Chemical | ChemComp-SO4 / |
#3: Chemical | ChemComp-HEM / |
#4: Chemical | ChemComp-PIY / |
#5: Water | ChemComp-HOH / |
Nonpolymer details | SOLVENT MOLECULE 515 IS VERY LIKELY A CATION. THE AUTHORS BASED THIS CONCLUSION ON THE OCTAHEDRAL ...SOLVENT MOLECULE 515 IS VERY LIKELY A CATION. THE AUTHORS BASED THIS CONCLUSION |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44.16 % | ||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 12-15 ℃ / pH: 7 / Method: unknown / Details: P-450CAM was prepared in solutions containing 1 | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
-Processing
Software | Name: PROLSQ / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Rfactor obs: 0.19 / Highest resolution: 1.6 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 1.6 Å
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Refine LS restraints |
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Software | *PLUS Name: PROLSQ / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.6 Å / Rfactor obs: 0.19 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |