+Open data
-Basic information
Entry | Database: PDB / ID: 1ous | ||||||
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Title | Lecb (PA-LII) calcium-free | ||||||
Components | hypothetical protein LecB | ||||||
Keywords | SUGAR BINDING PROTEIN / lectin / carbohydrate | ||||||
Function / homology | Function and homology information single-species biofilm formation / carbohydrate binding / metal ion binding Similarity search - Function | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.2 Å | ||||||
Authors | Loris, R. / Tielker, D. / Jaeger, K.-E. / Wyns, L. | ||||||
Citation | Journal: J.MOL.BIOL. / Year: 2003 Title: Structural Basis of Carbohydrate Recognition by the Lectin LecB from Pseudomonas aeruginosa Authors: Loris, R. / Tielker, D. / Jaeger, K.-E. / Wyns, L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ous.cif.gz | 101.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ous.ent.gz | 78.7 KB | Display | PDB format |
PDBx/mmJSON format | 1ous.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1ous_validation.pdf.gz | 447.9 KB | Display | wwPDB validaton report |
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Full document | 1ous_full_validation.pdf.gz | 454 KB | Display | |
Data in XML | 1ous_validation.xml.gz | 24.2 KB | Display | |
Data in CIF | 1ous_validation.cif.gz | 36.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ou/1ous ftp://data.pdbj.org/pub/pdb/validation_reports/ou/1ous | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 11734.707 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Gene: LecB / Plasmid: pET22b / Production host: Escherichia coli (E. coli) / References: UniProt: Q9HYN5 #2: Chemical | ChemComp-SO4 / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.63 Å3/Da / Density % sol: 24.04 % | |||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8 Details: PEG8000, ammonium sulphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K | |||||||||||||||
Crystal grow | *PLUS Method: microdialysis | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Beamline: X11 / Wavelength: 0.811 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Aug 12, 2002 / Details: mirrors |
Radiation | Monochromator: Si / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.811 Å / Relative weight: 1 |
Reflection | Resolution: 1.2→17 Å / Num. all: 104894 / Num. obs: 104894 / % possible obs: 95.7 % / Observed criterion σ(F): -3 / Observed criterion σ(I): -3 / Redundancy: 3.4 % / Rmerge(I) obs: 0.076 / Net I/σ(I): 9.35 |
Reflection shell | Resolution: 1.2→1.24 Å / Rmerge(I) obs: 0.185 / % possible all: 88.3 |
Reflection | *PLUS Num. obs: 104887 / Num. measured all: 356581 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.2→17 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.2→17 Å
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Refine LS restraints |
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Refinement | *PLUS | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS |