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- PDB-1om6: CRYSTAL STRUCTURE OF A COLD ADAPTED ALKALINE PROTEASE FROM PSEUDO... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1om6 | ||||||
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Title | CRYSTAL STRUCTURE OF A COLD ADAPTED ALKALINE PROTEASE FROM PSEUDOMONAS TAC II 18, CO-CRYSTALLIZED WITH 5mM EDTA (2 MONTHS) | ||||||
![]() | serralysin | ||||||
![]() | HYDROLASE / BETA JELLY ROLL | ||||||
Function / homology | ![]() serralysin / extracellular matrix / metalloendopeptidase activity / calcium ion binding / proteolysis / extracellular space / zinc ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ravaud, S. / Gouet, P. / Haser, R. / Aghajari, N. | ||||||
![]() | ![]() Title: Probing the role of divalent metal ions in a bacterial psychrophilic metalloprotease: binding studies of an enzyme in the crystalline state by x-ray crystallography. Authors: Ravaud, S. / Gouet, P. / Haser, R. / Aghajari, N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 109.1 KB | Display | ![]() |
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PDB format | ![]() | 81.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 435 KB | Display | ![]() |
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Full document | ![]() | 436.9 KB | Display | |
Data in XML | ![]() | 21.3 KB | Display | |
Data in CIF | ![]() | 32.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1o0qC ![]() 1o0tC ![]() 1om7C ![]() 1om8C ![]() 1omjC ![]() 1g9kS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 48727.609 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() | ||||
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#2: Chemical | ChemComp-CA / #3: Chemical | ChemComp-SO4 / | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 44.6 % | ||||||||||||||||||||||||
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 7 Details: Ammonium sulfate, Hepes, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 289K | ||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 7 / Method: vapor diffusion, hanging drop / Details: Villeret, V., (1997) Protein Sci., 6, 2462. | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | |||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||
Detector | Type: MARRESEARCH / Detector: CCD / Date: Apr 4, 2002 | |||||||||
Radiation | Monochromator: crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 2→34.1 Å / Num. all: 30649 / Num. obs: 30649 / % possible obs: 100 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 4 % / Biso Wilson estimate: 13.25 Å2 / Rsym value: 0.049 | |||||||||
Reflection shell | Resolution: 2→2.11 Å / Redundancy: 3.9 % / Rsym value: 0.167 / % possible all: 100 | |||||||||
Reflection | *PLUS Num. measured all: 123884 / Rmerge(I) obs: 0.081 | |||||||||
Reflection shell | *PLUS % possible obs: 100 % / Rmerge(I) obs: 0.167 / Mean I/σ(I) obs: 3.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1G9K Resolution: 2→50 Å / σ(F): 2 / σ(I): 2 / Stereochemistry target values: ENGH & HUBER
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Refinement step | Cycle: LAST / Resolution: 2→50 Å
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Refine LS restraints |
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