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Open data
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Basic information
Entry | Database: PDB / ID: 1akl | ||||||
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Title | ALKALINE PROTEASE FROM PSEUDOMONAS AERUGINOSA IFO3080 | ||||||
![]() | ALKALINE PROTEASE | ||||||
![]() | HYDROLASE (METALLOPROTEINASE) | ||||||
Function / homology | ![]() serralysin / negative regulation of complement activation, lectin pathway / negative regulation of complement activation, classical pathway / positive regulation of sodium ion transport / metalloendopeptidase activity / peptidase activity / calcium ion binding / proteolysis / extracellular space / zinc ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Miyatake, H. / Hata, Y. / Fujii, T. / Hamada, K. / Morihara, K. / Katsube, Y. | ||||||
![]() | ![]() Title: Crystal structure of the unliganded alkaline protease from Pseudomonas aeruginosa IFO3080 and its conformational changes on ligand binding. Authors: Miyatake, H. / Hata, Y. / Fujii, T. / Hamada, K. / Morihara, K. / Katsube, Y. #1: ![]() Title: Crystal Structure Determination of Alkaline Protease from Pseudomonas Aeruginosa Strain Pao1 Authors: Miyatake, H. / Hata, Y. / Fujii, T. / Morihara, K. / Katsube, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 108.8 KB | Display | ![]() |
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PDB format | ![]() | 82.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 362.3 KB | Display | ![]() |
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Full document | ![]() | 367.7 KB | Display | |
Data in XML | ![]() | 10.4 KB | Display | |
Data in CIF | ![]() | 17.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 49531.426 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() | ||
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#2: Chemical | ChemComp-ZN / | ||
#3: Chemical | ChemComp-CA / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.52 Å3/Da / Density % sol: 65.03 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal | *PLUS Density % sol: 66 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 25 K / pH: 7 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
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Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: May 25, 1993 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2→50 Å / Num. obs: 44289 / Observed criterion σ(I): 1 / Rmerge(I) obs: 0.058 |
Reflection | *PLUS Rmerge(I) obs: 0.058 |
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Processing
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Refinement | Resolution: 2→8 Å / σ(F): 2
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Displacement parameters | Biso mean: 38.12 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.3 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→8 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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