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Open data
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Basic information
Entry | Database: PDB / ID: 1of5 | ||||||
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Title | Crystal structure of Mex67-Mtr2 | ||||||
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![]() | NUCLEAR TRANSPORT / TRANSPORT / MRNA TRANSPORT / NUCLEAR PROTEIN / LEUCINE- RICH REPEAT | ||||||
Function / homology | ![]() nuclear RNA export factor complex / nuclear pore central transport channel / Transport of Mature mRNA derived from an Intron-Containing Transcript / U4 snRNA binding / poly(A)+ mRNA export from nucleus / porin activity / ribosomal large subunit export from nucleus / U5 snRNA binding / ribosomal small subunit export from nucleus / U2 snRNA binding ...nuclear RNA export factor complex / nuclear pore central transport channel / Transport of Mature mRNA derived from an Intron-Containing Transcript / U4 snRNA binding / poly(A)+ mRNA export from nucleus / porin activity / ribosomal large subunit export from nucleus / U5 snRNA binding / ribosomal small subunit export from nucleus / U2 snRNA binding / U6 snRNA binding / mRNA export from nucleus / nuclear pore / U1 snRNA binding / mRNA binding / RNA binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fribourg, S. / Conti, E. | ||||||
![]() | ![]() Title: Structural similarity in the absence of sequence homology of the messenger RNA export factors Mtr2 and p15. Authors: Fribourg, S. / Conti, E. | ||||||
History |
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Remark 700 | SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 71 KB | Display | ![]() |
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PDB format | ![]() | 51.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 430.5 KB | Display | ![]() |
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Full document | ![]() | 444.6 KB | Display | |
Data in XML | ![]() | 13.8 KB | Display | |
Data in CIF | ![]() | 17.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 24916.484 Da / Num. of mol.: 1 / Fragment: NTF2-LIKE DOMAIN, RESIDUES 268-484 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() |
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#2: Protein | Mass: 20802.506 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() |
#3: Chemical | ChemComp-HG / |
#4: Water | ChemComp-HOH / |
Compound details | MEX67 INVOLVED IN THE EXPORT OF MRNA FROM THE NUCLEUS TO THE CYTOPLASM. MTR2 AFFECTS MRNA TRANSPORT ...MEX67 INVOLVED IN THE EXPORT OF MRNA FROM THE NUCLEUS TO THE CYTOPLASM. MTR2 AFFECTS MRNA TRANSPORT FROM THE NUCLEUS TO THE CYTOPLASM |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.91 Å3/Da / Density % sol: 35.64 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.5 Details: 100MM HEPES PH7.5, PEG 3000 20%, 0.2M CALCIUM ACETATE, pH 7.50 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / pH: 7.5 / Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→30 Å / Num. obs: 9718 / % possible obs: 95.6 % / Observed criterion σ(I): 2 / Redundancy: 3.7 % / Rmerge(I) obs: 0.069 / Net I/σ(I): 9.4 |
Reflection shell | Resolution: 2.8→2.95 Å / Redundancy: 2.7 % / Rmerge(I) obs: 0.37 / Mean I/σ(I) obs: 2 / % possible all: 78.5 |
Reflection | *PLUS Highest resolution: 2.8 Å / Lowest resolution: 30 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.069 |
Reflection shell | *PLUS Highest resolution: 2.8 Å / % possible obs: 78.5 % / Redundancy: 2.7 % / Rmerge(I) obs: 0.37 / Mean I/σ(I) obs: 2 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.8→30 Å
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Refine LS restraints |
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Xplor file |
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Refinement | *PLUS Lowest resolution: 30 Å / % reflection Rfree: 5 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |