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- PDB-1obj: Crystal structure of the T150A mutant of Malonamidase E2 from Bra... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1obj | ||||||
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Title | Crystal structure of the T150A mutant of Malonamidase E2 from Bradyrhizobium japonicum | ||||||
![]() | MALONAMIDASE E2 | ||||||
![]() | AMIDASE | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Shin, S. / Oh, B.-H. | ||||||
![]() | ![]() Title: Characterization of a Novel Ser-Cisser-Lys Catalytic Triad in Comparison with the Classical Ser-His-Asp Triad Authors: Shin, S. / Yun, Y.S. / Koo, H.M. / Kim, Y.S. / Choi, K.Y. / Oh, B.-H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 179.1 KB | Display | ![]() |
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PDB format | ![]() | 142.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1o9nC ![]() 1o9oC ![]() 1o9pC ![]() 1o9qC ![]() 1obiC ![]() 1obkC ![]() 1oblC ![]() 1ochC ![]() 1ockC ![]() 1oclC ![]() 1ocmC ![]() 1gr8 C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 43705.727 Da / Num. of mol.: 2 / Mutation: YES Source method: isolated from a genetically manipulated source Details: CIS PEPTIDE BOND BETWEEN GLYCINE 130 AND SERINE 131 Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | Compound details | ENGINEERED | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 39.9 % |
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Crystal grow | pH: 7 / Details: 20% POLYETHYLENE GLYCOL, 0.1 M TRIS-HCL, PH 7.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU IMAGE PLATE / Detector: IMAGE PLATE / Details: MIRRORS |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→20 Å / Num. obs: 58083 / % possible obs: 91.7 % / Observed criterion σ(I): 1 / Redundancy: 4.5 % / Rsym value: 0.078 / Net I/σ(I): 13 |
Reflection shell | Resolution: 1.9→1.97 Å / Redundancy: 2.5 % / Mean I/σ(I) obs: 2.68 / Rsym value: 0.281 / % possible all: 81.3 |
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Processing
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Refinement | Method to determine structure: DIRECT METHODS Starting model: PDB ENTRY 1GR8 ![]() 1gr8 Resolution: 1.9→20 Å / Cross valid method: THROUGHOUT / σ(F): 1 Details: CIS PEPTIDE BOND BETWEEN GLYCINE 130 AND SERINE 131
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Refinement step | Cycle: LAST / Resolution: 1.9→20 Å
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Refine LS restraints |
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Xplor file |
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