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Open data
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Basic information
Entry | Database: PDB / ID: 1lbq | ||||||
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Title | The crystal structure of Saccharomyces cerevisiae ferrochelatase | ||||||
![]() | Ferrochelatase![]() | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Karlberg, T. / Lecerof, D. / Gora, M. / Silvegren, G. / Labbe-Bois, R. / Hansson, M. / Al-Karadaghi, S. | ||||||
![]() | ![]() Title: Metal binding to Saccharomyces cerevisiae ferrochelatase Authors: Karlberg, T. / Lecerof, D. / Gora, M. / Silvegren, G. / Labbe-Bois, R. / Hansson, M. / Al-Karadaghi, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 151 KB | Display | ![]() |
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PDB format | ![]() | 119.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1l8xC ![]() 1hrkS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 41000.727 Da / Num. of mol.: 2 / Fragment: protoheme ferrolyase Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Production host: ![]() ![]() ![]() #2: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.12 Å3/Da / Density % sol: 60.55 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: Peg2000, 2-propanol, Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 8 | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Nov 11, 2001 / Details: mirrors |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.4→19.8 Å / Num. all: 40095 / Num. obs: 40095 / % possible obs: 92.5 % / Observed criterion σ(F): 3 / Biso Wilson estimate: 45.3 Å2 / Rmerge(I) obs: 0.105 |
Reflection shell | Resolution: 2.4→2.5 Å / Num. unique all: 4893 / % possible all: 80.9 |
Reflection | *PLUS Lowest resolution: 10 Å / Num. obs: 37088 |
Reflection shell | *PLUS % possible obs: 80.9 % / Rmerge(I) obs: 0.199 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: human ferrochelatase, pdb code: 1hrk Resolution: 2.4→19.8 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.4→19.8 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.4→2.5 Å
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Refinement | *PLUS Lowest resolution: 10 Å / % reflection Rfree: 5 % | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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