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Yorodumi- PDB-5z06: Crystal structure of beta-1,2-glucanase from Parabacteroides dist... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5z06 | ||||||
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Title | Crystal structure of beta-1,2-glucanase from Parabacteroides distasonis | ||||||
Components | BDI_3064 protein | ||||||
Keywords | HYDROLASE / Sophorose / Parabacteroides / GH144 / Glycoside hydrolase / beta-1 / 2-glucanase / 2-glucan / 2-glucooligosaccharide | ||||||
Function / homology | exo beta-1,2-glucooligosaccharide sophorohydrolase (non-reducing end) / Glycoamylase-like, conserved domain / Putative glucoamylase / hydrolase activity, acting on glycosyl bonds / periplasmic space / Immunoglobulin-like fold / Exo beta-1,2-glucooligosaccharide sophorohydrolase (non-reducing end) Function and homology information | ||||||
Biological species | Parabacteroides distasonis ATCC 8503 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.1 Å | ||||||
Authors | Shimizu, H. / Nakajima, M. / Miyanaga, A. / Takahashi, Y. / Tanaka, N. / Kobayashi, K. / Sugimoto, N. / Nakai, H. / Taguchi, H. | ||||||
Funding support | Japan, 1items
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Citation | Journal: Biochemistry / Year: 2018 Title: Characterization and Structural Analysis of a Novel exo-Type Enzyme Acting on beta-1,2-Glucooligosaccharides from Parabacteroides distasonis Authors: Shimizu, H. / Nakajima, M. / Miyanaga, A. / Takahashi, Y. / Tanaka, N. / Kobayashi, K. / Sugimoto, N. / Nakai, H. / Taguchi, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5z06.cif.gz | 300.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5z06.ent.gz | 238.8 KB | Display | PDB format |
PDBx/mmJSON format | 5z06.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 5z06_validation.pdf.gz | 453.1 KB | Display | wwPDB validaton report |
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Full document | 5z06_full_validation.pdf.gz | 460.9 KB | Display | |
Data in XML | 5z06_validation.xml.gz | 52.3 KB | Display | |
Data in CIF | 5z06_validation.cif.gz | 75.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/z0/5z06 ftp://data.pdbj.org/pub/pdb/validation_reports/z0/5z06 | HTTPS FTP |
-Related structure data
Related structure data | 4qt9S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 81944.727 Da / Num. of mol.: 2 / Fragment: UNP residues 19-721 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Parabacteroides distasonis ATCC 8503 (bacteria) Strain: ATCC 8503 / Gene: BDI_3064 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: A6LGF6 #2: Chemical | #3: Chemical | ChemComp-GOL / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.91 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 8 Details: 200mM calcium acetate, 100mM imidazole/ hydrochloric acid pH 8.0, and 20%(w/v) PEG 1000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: AR-NW12A / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: Jun 2, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→46.73 Å / Num. obs: 86780 / % possible obs: 97.9 % / Redundancy: 3.9 % / Rmerge(I) obs: 0.068 / Net I/σ(I): 14.1 |
Reflection shell | Resolution: 2.1→2.14 Å / Redundancy: 2.8 % / Rmerge(I) obs: 0.57 / Mean I/σ(I) obs: 2.2 / Num. unique obs: 4410 / CC1/2: 0.739 / % possible all: 97.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4QT9 Resolution: 2.1→46.73 Å / Cor.coef. Fo:Fc: 0.958 / Cor.coef. Fo:Fc free: 0.933 / SU B: 5.195 / SU ML: 0.13 / Cross valid method: FREE R-VALUE / ESU R: 0.224 / ESU R Free: 0.179 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 33.02 Å2
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Refinement step | Cycle: 1 / Resolution: 2.1→46.73 Å
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Refine LS restraints |
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