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- PDB-1oao: NiZn[Fe4S4] and NiNi[Fe4S4] clusters in closed and open alpha sub... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1oao | ||||||
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Title | NiZn[Fe4S4] and NiNi[Fe4S4] clusters in closed and open alpha subunits of acetyl-CoA synthase/carbon monoxide dehydrogenase | ||||||
![]() | (CARBON MONOXIDE DEHYDROGENASE/ACETYL-COA SYNTHASE SUBUNIT ...) x 2 | ||||||
![]() | OXIDOREDUCTASE/TRANSFERASE / OXIDOREDUCTASE-TRANSFERASE COMPLEX / ELECTRON TRANSFER / OXIDOREDUCTASE / ACETYL-COA FORMATION / WOOD/LJUNGDAHL PATHWAY / NICKEL | ||||||
Function / homology | ![]() CO-methylating acetyl-CoA synthase / CO-methylating acetyl-CoA synthase activity / anaerobic carbon monoxide dehydrogenase / anaerobic carbon-monoxide dehydrogenase activity / : / acetyl-CoA metabolic process / hydroxylamine reductase activity / carbon fixation / nickel cation binding / generation of precursor metabolites and energy ...CO-methylating acetyl-CoA synthase / CO-methylating acetyl-CoA synthase activity / anaerobic carbon monoxide dehydrogenase / anaerobic carbon-monoxide dehydrogenase activity / : / acetyl-CoA metabolic process / hydroxylamine reductase activity / carbon fixation / nickel cation binding / generation of precursor metabolites and energy / response to hydrogen peroxide / peroxidase activity / 4 iron, 4 sulfur cluster binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Darnault, C. / Volbeda, A. / Kim, E.J. / Legrand, P. / Vernede, X. / Lindahl, P.A. / Fontecilla-Camps, J.C. | ||||||
![]() | ![]() Title: Ni-Zn-[Fe4-S4] and Ni-Ni-[Fe4-S4] Clusters in Closed and Open Alpha Subunits of Acetyl-Coa Synthase/Carbon Monoxide Dehydrogenase Authors: Darnault, C. / Volbeda, A. / Kim, E.J. / Legrand, P. / Vernede, X. / Lindahl, P.A. / Fontecilla-Camps, J.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 573.5 KB | Display | ![]() |
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PDB format | ![]() | 467.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 571.1 KB | Display | ![]() |
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Full document | ![]() | 631.7 KB | Display | |
Data in XML | ![]() | 111.9 KB | Display | |
Data in CIF | ![]() | 161 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1jjy S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.161329, -0.978881, -0.125561), Vector: |
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Components
-CARBON MONOXIDE DEHYDROGENASE/ACETYL-COA SYNTHASE SUBUNIT ... , 2 types, 4 molecules ABCD
#1: Protein | Mass: 73006.930 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() References: UniProt: P27989, carbon-monoxide dehydrogenase (acceptor), anaerobic carbon monoxide dehydrogenase #2: Protein | Mass: 81816.930 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() References: UniProt: P27988, CO-methylating acetyl-CoA synthase |
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-Non-polymers , 12 types, 1328 molecules ![](data/chem/img/SF4.gif)
![](data/chem/img/XCC.gif)
![](data/chem/img/FOR.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/FE2.gif)
![](data/chem/img/BCT.gif)
![](data/chem/img/ZN.gif)
![](data/chem/img/NI.gif)
![](data/chem/img/SX.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/XCC.gif)
![](data/chem/img/FOR.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/FE2.gif)
![](data/chem/img/BCT.gif)
![](data/chem/img/ZN.gif)
![](data/chem/img/NI.gif)
![](data/chem/img/SX.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-SF4 / #4: Chemical | #5: Chemical | #6: Chemical | ChemComp-SO4 / #7: Chemical | ChemComp-GOL / #8: Chemical | #9: Chemical | ChemComp-BCT / | #10: Chemical | ChemComp-ZN / | #11: Chemical | #12: Chemical | ChemComp-SX / | #13: Chemical | ChemComp-ACT / | #14: Water | ChemComp-HOH / | |
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-Details
Compound details | CATALYZES THE INTERCONVERSION OF CO AND CO2 AND THE SYNTHESIS OF ACETYL-COENZYME A FROM THE ...CATALYZES THE INTERCONVE |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.69 Å3/Da / Density % sol: 54.24 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.2 / Details: pH 7.20 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 8 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Sep 15, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→29.2 Å / Num. obs: 479707 / % possible obs: 94.4 % / Redundancy: 1.8 % / Rmerge(I) obs: 0.053 |
Reflection shell | Resolution: 1.9→1.95 Å / Redundancy: 1.5 % / Rmerge(I) obs: 0.238 / % possible all: 67.9 |
Reflection | *PLUS Highest resolution: 1.9 Å / Lowest resolution: 30 Å / Num. obs: 239946 |
Reflection shell | *PLUS % possible obs: 67.9 % / Mean I/σ(I) obs: 3.3 |
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Processing
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Refinement | Method to determine structure: ![]() ![]() Starting model: PDB ENTRY 1JJY ![]() 1jjy Resolution: 1.9→25 Å / Cor.coef. Fo:Fc: 0.967 / Cor.coef. Fo:Fc free: 0.952 / SU B: 2.296 / SU ML: 0.068 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / ESU R: 0.114 / ESU R Free: 0.108 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 12.79 Å2
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Refinement step | Cycle: LAST / Resolution: 1.9→25 Å
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Refine LS restraints |
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