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- PDB-1o9f: Structural view of a fungal toxin acting on a 14-3-3 regulatory c... -

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Basic information

Entry
Database: PDB / ID: 1o9f
TitleStructural view of a fungal toxin acting on a 14-3-3 regulatory complex
Components
  • 14-3-3-LIKE PROTEIN C
  • PLASMA MEMBRANE H+ ATPASE
KeywordsPROTEIN BINDING / PROTEIN-BINDING / FUSICOCCIN / 14-3-3 FAMILY / ACTIVATING DRUG / PLANT PLASMA MEMBRANE (H+)ATPASE
Function / homology
Function and homology information


protein localization => GO:0008104 / ATP hydrolysis activity / signal transduction / integral component of membrane / ATP binding / cytoplasm
Similarity search - Function
14-3-3 domain / Delta-Endotoxin; domain 1 / 14-3-3 proteins signature 2. / 14-3-3 proteins signature 1. / 14-3-3 protein, conserved site / 14-3-3 homologues / 14-3-3 protein / 14-3-3 domain / 14-3-3 domain superfamily / 14-3-3 protein ...14-3-3 domain / Delta-Endotoxin; domain 1 / 14-3-3 proteins signature 2. / 14-3-3 proteins signature 1. / 14-3-3 protein, conserved site / 14-3-3 homologues / 14-3-3 protein / 14-3-3 domain / 14-3-3 domain superfamily / 14-3-3 protein / P-type ATPase / P-type ATPase, transmembrane domain superfamily / HAD superfamily / HAD-like superfamily / Up-down Bundle / Mainly Alpha
Similarity search - Domain/homology
FUSICOCCIN / 14-3-3-like protein C / Plasma membrane H+ ATPase
Similarity search - Component
Biological speciesNICOTIANA TABACUM (common tobacco)
NICOTIANA PLUMBAGINIFOLIA (curled-leaved tobacco)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.7 Å
AuthorsWurtele, M. / Jelich-Ottmann, C. / Wittinghofer, A. / Oecking, C.
CitationJournal: Embo J. / Year: 2003
Title: Structural View of a Fungal Toxin Acting on a 14-3-3 Regulatory Complex
Authors: Wurtele, M. / Jelich-Ottmann, C. / Wittinghofer, A. / Oecking, C.
History
DepositionDec 12, 2002Deposition site: PDBE / Processing site: PDBE
Revision 1.0Mar 6, 2003Provider: repository / Type: Initial release
Revision 1.1May 8, 2011Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: 14-3-3-LIKE PROTEIN C
P: PLASMA MEMBRANE H+ ATPASE
hetero molecules


Theoretical massNumber of molelcules
Total (without water)30,7573
Polymers30,0772
Non-polymers6811
Water1,11762
1
A: 14-3-3-LIKE PROTEIN C
P: PLASMA MEMBRANE H+ ATPASE
hetero molecules

A: 14-3-3-LIKE PROTEIN C
P: PLASMA MEMBRANE H+ ATPASE
hetero molecules


Theoretical massNumber of molelcules
Total (without water)61,5156
Polymers60,1534
Non-polymers1,3622
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation11_655-x+y+1,y,-z+1/21
Buried area6350 Å2
ΔGint-47.33 kcal/mol
Surface area22330 Å2
MethodPISA
Unit cell
Length a, b, c (Å)108.800, 108.800, 135.700
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number179
Space group name H-MP6522
Components on special symmetry positions
IDModelComponents
11A-2014-

HOH

DetailsTHE ENTRY IS A DIMER OF CHAIN A, WHICH BECOMES A TETRAMER AS PEPTIDE CHAIN P IS IN COMPLEX WITH CHAIN A.

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Components

#1: Protein 14-3-3-LIKE PROTEIN C


Mass: 29399.977 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: GENE BANK AAC49892 / Source: (gene. exp.) NICOTIANA TABACUM (common tobacco) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P93343
#2: Protein/peptide PLASMA MEMBRANE H+ ATPASE


Mass: 676.610 Da / Num. of mol.: 1 / Fragment: RESIDUES 436-440 / Source method: obtained synthetically
Source: (synth.) NICOTIANA PLUMBAGINIFOLIA (curled-leaved tobacco)
References: UniProt: Q40409
#3: Chemical ChemComp-FSC / FUSICOCCIN / Fusicoccin


Mass: 680.823 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C36H56O12
#4: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 62 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.85 Å3/Da / Density % sol: 68.09 %
Crystal growpH: 6.4 / Details: PEG400, CITRAT PH 4.7, 0.2 MM AMMONIUM ACETATE
Crystal grow
*PLUS
pH: 4.7 / Method: vapor diffusion, hanging drop
Components of the solutions
*PLUS
IDConc.Common nameCrystal-IDSol-IDDetails
121 %PEG4001reservoir
20.1 mMcitrate1reservoirpH4.7
30.2 mMammonium acetate1reservoirpH7.0
410 mMdithiothreitol1reservoir

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: ID29 / Wavelength: 0.979
DetectorDate: Dec 15, 2001
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979 Å / Relative weight: 1
ReflectionResolution: 2.7→10 Å / Num. obs: 13170 / % possible obs: 96.8 % / Observed criterion σ(I): 2 / Redundancy: 4.3 % / Biso Wilson estimate: 47.6 Å2 / Rmerge(I) obs: 0.066 / Net I/σ(I): 14
Reflection shellResolution: 2.7→2.8 Å / Rmerge(I) obs: 0.3 / Mean I/σ(I) obs: 4.2 / % possible all: 97.2
Reflection
*PLUS
Lowest resolution: 10 Å / Num. measured all: 56531
Reflection shell
*PLUS
% possible obs: 97.2 % / Rmerge(I) obs: 0.3

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Processing

Software
NameVersionClassification
CNS1.1refinement
XDSdata reduction
XDSdata scaling
AMoREphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 1A4O
Resolution: 2.7→19.21 Å / Rfactor Rfree error: 0.01 / Data cutoff high absF: 541374.11 / Cross valid method: THROUGHOUT / σ(F): 0
RfactorNum. reflection% reflectionSelection details
Rfree0.263 658 5 %RANDOM
Rwork0.224 ---
obs0.224 13179 97.3 %-
Solvent computationSolvent model: FLAT MODEL / Bsol: 49.7886 Å2 / ksol: 0.352667 e/Å3
Displacement parametersBiso mean: 60.4 Å2
Baniso -1Baniso -2Baniso -3
1-11.15 Å28.87 Å20 Å2
2--11.15 Å20 Å2
3----22.3 Å2
Refine analyze
FreeObs
Luzzati coordinate error0.4 Å0.36 Å
Luzzati d res low-5 Å
Luzzati sigma a0.38 Å0.39 Å
Refinement stepCycle: LAST / Resolution: 2.7→19.21 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms1892 0 48 62 2002
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONc_bond_d0.007
X-RAY DIFFRACTIONc_bond_d_na
X-RAY DIFFRACTIONc_bond_d_prot
X-RAY DIFFRACTIONc_angle_d
X-RAY DIFFRACTIONc_angle_d_na
X-RAY DIFFRACTIONc_angle_d_prot
X-RAY DIFFRACTIONc_angle_deg1.2
X-RAY DIFFRACTIONc_angle_deg_na
X-RAY DIFFRACTIONc_angle_deg_prot
X-RAY DIFFRACTIONc_dihedral_angle_d18.6
X-RAY DIFFRACTIONc_dihedral_angle_d_na
X-RAY DIFFRACTIONc_dihedral_angle_d_prot
X-RAY DIFFRACTIONc_improper_angle_d0.82
X-RAY DIFFRACTIONc_improper_angle_d_na
X-RAY DIFFRACTIONc_improper_angle_d_prot
X-RAY DIFFRACTIONc_mcbond_it
X-RAY DIFFRACTIONc_mcangle_it
X-RAY DIFFRACTIONc_scbond_it
X-RAY DIFFRACTIONc_scangle_it
LS refinement shellResolution: 2.7→2.87 Å / Rfactor Rfree error: 0.034 / Total num. of bins used: 5
RfactorNum. reflection% reflection
Rfree0.352 106 4.9 %
Rwork0.305 2407 -
obs--97.6 %
Refinement
*PLUS
Lowest resolution: 10 Å / % reflection Rfree: 5 %
Solvent computation
*PLUS
Displacement parameters
*PLUS
Refine LS restraints
*PLUS
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONc_bond_d0.008
X-RAY DIFFRACTIONc_dihedral_angle_d
X-RAY DIFFRACTIONc_dihedral_angle_deg18.6
X-RAY DIFFRACTIONc_improper_angle_d
X-RAY DIFFRACTIONc_improper_angle_deg0.82

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