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- PDB-1o6o: Importin Beta aa1-442 bound to five FxFG repeats from yeast Nsp1p... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1o6o | ||||||
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Title | Importin Beta aa1-442 bound to five FxFG repeats from yeast Nsp1p. Second crystal form | ||||||
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![]() | NUCLEAR TRANSPORT / NUCLEAR TRAFFICKING / NUCLEOPORIN / TRANSPORT FACTOR / PROTEIN TRANSPORT | ||||||
Function / homology | ![]() RNA import into nucleus / Inhibition of nitric oxide production / mitotic chromosome movement towards spindle pole / endoplasmic reticulum tubular network / establishment of mitotic spindle localization / astral microtubule organization / nuclear pore central transport channel / Transport of Mature mRNA derived from an Intron-Containing Transcript / Regulation of HSF1-mediated heat shock response / nuclear pore nuclear basket ...RNA import into nucleus / Inhibition of nitric oxide production / mitotic chromosome movement towards spindle pole / endoplasmic reticulum tubular network / establishment of mitotic spindle localization / astral microtubule organization / nuclear pore central transport channel / Transport of Mature mRNA derived from an Intron-Containing Transcript / Regulation of HSF1-mediated heat shock response / nuclear pore nuclear basket / Transport of Ribonucleoproteins into the Host Nucleus / Regulation of cholesterol biosynthesis by SREBP (SREBF) / SUMOylation of SUMOylation proteins / importin-alpha family protein binding / tRNA export from nucleus / ribosomal protein import into nucleus / Initiation of Nuclear Envelope (NE) Reformation / NS1 Mediated Effects on Host Pathways / NLS-dependent protein nuclear import complex / Apoptosis induced DNA fragmentation / SUMOylation of RNA binding proteins / structural constituent of nuclear pore / Nuclear import of Rev protein / RNA export from nucleus / SUMOylation of chromatin organization proteins / Postmitotic nuclear pore complex (NPC) reformation / nucleocytoplasmic transport / nuclear import signal receptor activity / poly(A)+ mRNA export from nucleus / nuclear localization sequence binding / mitotic metaphase chromosome alignment / NLS-bearing protein import into nucleus / ribosomal large subunit export from nucleus / mitotic spindle assembly / nuclear pore / ribosomal small subunit export from nucleus / Assembly of the ORC complex at the origin of replication / nuclear periphery / Hsp90 protein binding / phospholipid binding / ISG15 antiviral mechanism / small GTPase binding / cytoplasmic stress granule / specific granule lumen / protein import into nucleus / SARS-CoV-1 activates/modulates innate immune responses / Interferon alpha/beta signaling / nuclear envelope / nuclear membrane / ficolin-1-rich granule lumen / protein domain specific binding / Neutrophil degranulation / enzyme binding / RNA binding / extracellular exosome / zinc ion binding / extracellular region / nucleoplasm / membrane / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Bayliss, R. / Stewart, M. | ||||||
![]() | ![]() Title: Glfg and Fxfg Nucleoporins Bind to Overlapping Sites on Importin-Beta Authors: Bayliss, R. / Littlewood, T. / Strawn, L.A. / Wente, S.R. / Stewart, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 265.4 KB | Display | ![]() |
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PDB format | ![]() | 214.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1o6pC ![]() 1qgkS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 49385.203 Da / Num. of mol.: 3 / Fragment: RESIDUES 1-442 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 12553.628 Da / Num. of mol.: 3 / Fragment: RESIDUES 497-608 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() #3: Water | ChemComp-HOH / | Compound details | IMPORTIN BETA-1 ROLE IN NUCLEAR PROTEIN IMPORT, RECEPTOR FOR NUCLEAR LOCALIZATION SIGNALS. MEMBER ...IMPORTIN BETA-1 ROLE IN NUCLEAR PROTEIN IMPORT, RECEPTOR FOR NUCLEAR LOCALIZATI | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.02 Å3/Da / Density % sol: 59.25 % |
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Crystal grow | pH: 5.9 Details: 1.2-1.28M AMMONIUM SULPHATE, 100MM AMMONIUM ACETATE, PH 5.9, 30MM DTT |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→60.9 Å / Num. obs: 53288 / % possible obs: 94.7 % / Redundancy: 3.9 % / Rmerge(I) obs: 0.092 / Net I/σ(I): 5.5 |
Reflection shell | Resolution: 2.8→2.95 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.387 / Mean I/σ(I) obs: 1.7 / % possible all: 88.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1QGK Resolution: 2.8→20 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.8→20 Å
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Refine LS restraints |
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