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Yorodumi- PDB-1no4: Crystal Structure of the pre-assembly scaffolding protein gp7 fro... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1no4 | ||||||
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Title | Crystal Structure of the pre-assembly scaffolding protein gp7 from the double-stranded DNA bacteriophage phi29 | ||||||
Components | HEAD MORPHOGENESIS PROTEIN | ||||||
Keywords | VIRAL PROTEIN / coiled-coil | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Bacillus phage phi29 (virus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.2 Å | ||||||
Authors | Morais, M.C. / Kanamaru, S. / Badasso, M.O. / Koti, J.S. / Owen, B.A.L. / McMurray, C.T. / Anderson, D.L. / Rossmann, M.G. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2003 Title: Bacteriophage f29 scaffolding protein gp7 before and after prohead assembly Authors: Morais, M.C. / Kanamaru, S. / Badasso, M.O. / Koti, J.S. / Owen, B.A.L. / McMurray, C.T. / Anderson, D.L. / Rossmann, M.G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1no4.cif.gz | 72.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1no4.ent.gz | 55.4 KB | Display | PDB format |
PDBx/mmJSON format | 1no4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/no/1no4 ftp://data.pdbj.org/pub/pdb/validation_reports/no/1no4 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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Details | The biological unit is a dimeric coiled-coil. There are two such units per asymmetric unit. |
-Components
#1: Protein | Mass: 11165.360 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus phage phi29 (virus) / Genus: Phi29-like viruses / Gene: gp7 / Plasmid: pARgp7 / Production host: Escherichia coli (E. coli) / Strain (production host): HMS174(DE3) / References: UniProt: P13848 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 5.56 Å3/Da / Density % sol: 77.7 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 4 % PEG 8000, 0.16 M calcium acetate, 0.08 M sodium cacodylate, 20 % glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: unknown | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 14-BM-C / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Nov 17, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→60.3 Å / Num. all: 38534 / Num. obs: 38534 / % possible obs: 95.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 20.06 % / Biso Wilson estimate: 32.8 Å2 / Rsym value: 0.068 / Net I/σ(I): 19.5 |
Reflection shell | Resolution: 2.2→2.26 Å / Mean I/σ(I) obs: 5 / Num. unique all: 1495 / Rsym value: 0.255 / % possible all: 75.4 |
Reflection | *PLUS Highest resolution: 2.2 Å / Num. obs: 40285 / Rmerge(I) obs: 0.068 |
Reflection shell | *PLUS % possible obs: 75.4 % / Rmerge(I) obs: 0.251 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 2.2→60.3 Å / Rfactor Rfree error: 0.004 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 58.7035 Å2 / ksol: 0.384725 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso mean: 58.3 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.2→60.3 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.34 Å / Rfactor Rfree error: 0.015 / Total num. of bins used: 6
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Xplor file |
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Refinement | *PLUS Highest resolution: 2.2 Å | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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