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- PDB-1nmb: THE STRUCTURE OF A COMPLEX BETWEEN THE NC10 ANTIBODY AND INFLUENZ... -
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Basic information
Entry | Database: PDB / ID: 1nmb | |||||||||
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Title | THE STRUCTURE OF A COMPLEX BETWEEN THE NC10 ANTIBODY AND INFLUENZA VIRUS NEURAMINIDASE AND COMPARISON WITH THE OVERLAPPING BINDING SITE OF THE NC41 ANTIBODY | |||||||||
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![]() | COMPLEX (HYDROLASE/IMMUNOGLOBULIN) / COMPLEX (HYDROLASE-IMMUNOGLOBULIN) / COMPLEX (HYDROLASE-IMMUNOGLOBULIN) complex | |||||||||
Function / homology | ![]() exo-alpha-(2->3)-sialidase activity / exo-alpha-(2->6)-sialidase activity / exo-alpha-(2->8)-sialidase activity / exo-alpha-sialidase / viral budding from plasma membrane / carbohydrate metabolic process / host cell plasma membrane / virion membrane / membrane / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() | |||||||||
![]() | Malby, R.L. / Tulip, W.R. / Colman, P.M. | |||||||||
![]() | ![]() Title: The structure of a complex between the NC10 antibody and influenza virus neuraminidase and comparison with the overlapping binding site of the NC41 antibody Authors: Malby, R.L. / Tulip, W.R. / Harley, V.R. / McKimm-Breschkin, J.L. / Laver, W.G. / Webster, R.G. / Colman, P.M. #1: ![]() Title: Three-Dimensional Structures of Influenza Virus Neuraminidase-Antibody Complexes Authors: Colman, P.M. / Tulip, W.R. / Varghese, J.N. / Tulloch, P.A. / Baker, A.T. / Laver, W.G. / Air, G.M. / Webster, R.G. #2: ![]() Title: Crystal Structure of Neuraminidase-Antibody Complexes Authors: Tulip, W.R. / Varghese, J.N. / Webster, R.G. / Air, G.M. / Laver, W.G. / Colman, P.M. #3: ![]() Title: N9 Neuraminidase Complexes with Antibodies Nc41 and Nc10: Empirical Free Energy Calculations Capture Specificity Trends Observed with Mutant Binding Data Authors: Tulip, W.R. / Harley, V.R. / Webster, R.G. / Novotny, J. #4: ![]() Title: Refined Crystal Structure of the Influenza Virus N9 Neuraminidase-Nc41 Complex Authors: Tulip, W.R. / Varghese, J.N. / Laver, W.G. / Webster, R.G. / Colman, P.M. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 135.1 KB | Display | ![]() |
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PDB format | ![]() | 106.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO N 326 / 2: CIS PROLINE - PRO N 431 / 3: CIS PROLINE - PRO L 95 |
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Components
-Protein , 1 types, 1 molecules N
#1: Protein | Mass: 52442.445 Da / Num. of mol.: 1 / Mutation: WILD TYPE / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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-Antibody , 2 types, 2 molecules LH
#2: Antibody | Mass: 12129.266 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: ISOLATED FROM MONOCLONAL MURINE ANTIBODY / Source: (natural) ![]() ![]() |
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#3: Antibody | Mass: 13423.723 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: ISOLATED FROM MONOCLONAL MURINE ANTIBODY / Source: (natural) ![]() ![]() |
-Sugars , 2 types, 3 molecules ![](data/chem/img/NAG.gif)
#4: Polysaccharide | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
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#5: Sugar |
-Non-polymers , 2 types, 84 molecules ![](data/chem/img/CA.gif)
![](data/chem/img/HOH.gif)
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#6: Chemical | ChemComp-CA / |
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#7: Water | ChemComp-HOH / |
-Details
Nonpolymer details | 83 SOLVENT ATOMS AND 1 CALCIUM ATOM INCLUDED IN MODEL. |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.61 Å3/Da / Density % sol: 65.89 % | ||||||||||||||||||||
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Crystal grow | *PLUS pH: 6.6 / Method: vapor diffusion | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.2 Å / Num. obs: 31862 / Observed criterion σ(F): 2 / Num. measured all: 176473 / Rmerge(I) obs: 0.095 |
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Processing
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Refinement | Resolution: 2.2→6 Å / σ(F): 2 Details: THE WHOLE OF THE CONSTANT MODULE OF THE FAB (CL AND CH1) IS NOT OBSERVED IN ELECTRON DENSITY MAPS AND IS PRESUMABLY DISORDERED. THE IDENTITY OF THR L 7 IS IN QUESTION AND IT COULD BE PRO. ...Details: THE WHOLE OF THE CONSTANT MODULE OF THE FAB (CL AND CH1) IS NOT OBSERVED IN ELECTRON DENSITY MAPS AND IS PRESUMABLY DISORDERED. THE IDENTITY OF THR L 7 IS IN QUESTION AND IT COULD BE PRO. THEREFORE IT IS ASSIGNED AN OCCUPANCY OF 0.0.
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Refinement step | Cycle: LAST / Resolution: 2.2→6 Å
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Refine LS restraints |
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