+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1nau | ||||||
---|---|---|---|---|---|---|---|
タイトル | NMR Solution Structure of the Glucagon Antagonist [desHis1, desPhe6, Glu9]Glucagon Amide in the Presence of Perdeuterated Dodecylphosphocholine Micelles | ||||||
要素 | Glucagonグルカゴン | ||||||
キーワード | HORMONE/GROWTH FACTOR / HELIX (螺旋) / TURN (ターン) / HORMONE-GROWTH FACTOR COMPLEX | ||||||
機能・相同性 | 機能・相同性情報 glucagon receptor binding / : / negative regulation of execution phase of apoptosis / feeding behavior / positive regulation of calcium ion import / cellular response to glucagon stimulus / positive regulation of insulin secretion involved in cellular response to glucose stimulus / regulation of insulin secretion / Synthesis, secretion, and deacylation of Ghrelin / positive regulation of gluconeogenesis ...glucagon receptor binding / : / negative regulation of execution phase of apoptosis / feeding behavior / positive regulation of calcium ion import / cellular response to glucagon stimulus / positive regulation of insulin secretion involved in cellular response to glucose stimulus / regulation of insulin secretion / Synthesis, secretion, and deacylation of Ghrelin / positive regulation of gluconeogenesis / protein kinase A signaling / response to activity / positive regulation of peptidyl-threonine phosphorylation / 糖新生 / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / Glucagon signaling in metabolic regulation / adenylate cyclase-activating G protein-coupled receptor signaling pathway / hormone activity / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / Glucagon-type ligand receptors / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / positive regulation of peptidyl-serine phosphorylation / glucose homeostasis / G alpha (s) signalling events / G alpha (q) signalling events / secretory granule lumen / positive regulation of ERK1 and ERK2 cascade / G protein-coupled receptor signaling pathway / 小胞体 / signaling receptor binding / negative regulation of apoptotic process / extracellular space / extracellular region / identical protein binding / 細胞膜 類似検索 - 分子機能 | ||||||
手法 | 溶液NMR / distance geometry, restrained molecular dynamics | ||||||
Model type details | minimized average | ||||||
データ登録者 | Ying, J. / Ahn, J.-M. / Jacobsen, N.E. / Brown, M.F. / Hruby, V.J. | ||||||
引用 | ジャーナル: Biochemistry / 年: 2003 タイトル: NMR Solution Structure of the Glucagon Antagonist [desHis1, desPhe6, Glu9]Glucagon Amide in the Presence of Perdeuterated Dodecylphosphocholine Micelles 著者: Ying, J. / Ahn, J.-M. / Jacobsen, N.E. / Brown, M.F. / Hruby, V.J. | ||||||
履歴 |
|
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
---|
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1nau.cif.gz | 140 KB | 表示 | PDBx/mmCIF形式 |
---|---|---|---|---|
PDB形式 | pdb1nau.ent.gz | 120.8 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1nau.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/na/1nau ftp://data.pdbj.org/pub/pdb/validation_reports/na/1nau | HTTPS FTP |
---|
-関連構造データ
-リンク
-集合体
登録構造単位 |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR アンサンブル |
|
-要素
#1: タンパク質・ペプチド | 分子量: 3213.495 Da / 分子数: 1 / 変異: DEL(H53), DEL(F58), D7E / 由来タイプ: 合成 詳細: This sequence was chemically synthesized. It was obtained by modifying the sequence of glucagon, which occurs naturally in Homo sapiens (human). 参照: UniProt: P01275 |
---|
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR実験 |
| ||||||||||||||||
NMR実験の詳細 | Text: This structure was determined using standard 2D homonuclear techniques. |
-試料調製
詳細 | 内容: 7mM [desHis1, desPhe6, Glu9]Glucagon Amide; 50 mM sodium phosphate buffer; 283 mM dodecylphosphocholine-d38 micelles; 1 mM sodium azide; 90% H2O, 10% D2O 溶媒系: 90% H2O/10% D2O |
---|---|
試料状態 | イオン強度: 50 mM sodium phosphate; 1mM sodium azide / pH: 6.0 / 圧: ambient / 温度: 310 K |
結晶化 | *PLUS 手法: その他 / 詳細: NMR |
-NMR測定
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M |
---|---|
放射波長 | 相対比: 1 |
NMRスペクトロメーター | タイプ: Bruker AMX / 製造業者: Bruker / モデル: AMX / 磁場強度: 500 MHz |
-解析
NMR software |
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
精密化 | 手法: distance geometry, restrained molecular dynamics / ソフトェア番号: 1 詳細: The structures are based on a total of 486 restraints, 475 are NOE-derived distance restraints, 11 dihedral angle restraints | ||||||||||||||||||||||||
代表構造 | 選択基準: minimized average structure | ||||||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: 15 structures with the lowest restraint energy 計算したコンフォーマーの数: 50 / 登録したコンフォーマーの数: 16 |