- PDB-1nau: NMR Solution Structure of the Glucagon Antagonist [desHis1, desPh... -
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Basic information
Entry
Database: PDB / ID: 1nau
Title
NMR Solution Structure of the Glucagon Antagonist [desHis1, desPhe6, Glu9]Glucagon Amide in the Presence of Perdeuterated Dodecylphosphocholine Micelles
Mass: 3213.495 Da / Num. of mol.: 1 / Mutation: DEL(H53), DEL(F58), D7E / Source method: obtained synthetically Details: This sequence was chemically synthesized. It was obtained by modifying the sequence of glucagon, which occurs naturally in Homo sapiens (human). References: UniProt: P01275
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
DQF-COSY
1
2
1
2D TOCSY
1
3
1
2D NOESY
NMR details
Text: This structure was determined using standard 2D homonuclear techniques.
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Sample preparation
Details
Contents: 7mM [desHis1, desPhe6, Glu9]Glucagon Amide; 50 mM sodium phosphate buffer; 283 mM dodecylphosphocholine-d38 micelles; 1 mM sodium azide; 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O
Sample conditions
Ionic strength: 50 mM sodium phosphate; 1mM sodium azide / pH: 6.0 / Pressure: ambient / Temperature: 310 K
Crystal grow
*PLUS
Method: other / Details: NMR
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NMR measurement
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelength
Relative weight: 1
NMR spectrometer
Type: Bruker AMX / Manufacturer: Bruker / Model: AMX / Field strength: 500 MHz
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Processing
NMR software
Name
Version
Developer
Classification
UXNMR
Bruker
collection
Felix
2000
Accelrys
processing
Felix
2000
Accelrys
dataanalysis
DGII
withinInsightII2000
Accelrys
structuresolution
Discover
withinInsightII2000
Accelrys
refinement
Refinement
Method: distance geometry, restrained molecular dynamics / Software ordinal: 1 Details: The structures are based on a total of 486 restraints, 475 are NOE-derived distance restraints, 11 dihedral angle restraints
NMR representative
Selection criteria: minimized average structure
NMR ensemble
Conformer selection criteria: 15 structures with the lowest restraint energy Conformers calculated total number: 50 / Conformers submitted total number: 16
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