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- PDB-1n2z: 2.0 Angstrom structure of BtuF, the vitamin B12 binding protein o... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1n2z | |||||||||
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Title | 2.0 Angstrom structure of BtuF, the vitamin B12 binding protein of E. coli | |||||||||
![]() | Vitamin B12 transport protein btuF | |||||||||
![]() | TRANSPORT PROTEIN | |||||||||
Function / homology | ![]() cobalamin transport complex / cobalamin transport / cobalamin binding / outer membrane-bounded periplasmic space / periplasmic space / membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Borths, E.L. / Locher, K.P. / Lee, A.T. / Rees, D.C. | |||||||||
![]() | ![]() Title: The structure of Escherichia coli BtuF and binding to its cognate ATP binding cassette transporter Authors: Borths, E.L. / Locher, K.P. / Lee, A.T. / Rees, D.C. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 121.1 KB | Display | ![]() |
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PDB format | ![]() | 100.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 |
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4 | ![]()
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Unit cell |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 27046.812 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Non-polymers , 5 types, 408 molecules ![](data/chem/img/CD.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/CNC.gif)
![](data/chem/img/PG4.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/CNC.gif)
![](data/chem/img/PG4.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-CD / #3: Chemical | ChemComp-CL / #4: Chemical | #5: Chemical | ChemComp-PG4 / | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.2 Å3/Da / Density % sol: 61.51 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 4 ℃ / pH: 4.6 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. obs: 46346 / Observed criterion σ(I): -3 |
Reflection | *PLUS Highest resolution: 2 Å / Lowest resolution: 20 Å / % possible obs: 100 % / Redundancy: 10.1 % / Rmerge(I) obs: 0.058 |
Reflection shell | *PLUS % possible obs: 100 % / Rmerge(I) obs: 0.182 / Mean I/σ(I) obs: 11.1 |
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Processing
Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 2→20 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 20 Å / Rfactor Rfree: 0.21 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS |