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Yorodumi- PDB-1mwb: Solution structure of the recombinant hemoglobin from the cyanoba... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1mwb | ||||||
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Title | Solution structure of the recombinant hemoglobin from the cyanobacterium Synechocystis sp. PCC 6803 in its hemichrome state | ||||||
Components | Cyanoglobin | ||||||
Keywords | OXYGEN STORAGE/TRANSPORT / globin / cyanoglobin / OXYGEN STORAGE-TRANSPORT COMPLEX | ||||||
Function / homology | Function and homology information thioredoxin peroxidase activity / cell redox homeostasis / oxygen carrier activity / oxygen binding / cellular response to oxidative stress / heme binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Synechocystis sp. (bacteria) | ||||||
Method | SOLUTION NMR / distance geometry, simulated annealing, molecular dynamics, torsional angle database | ||||||
Authors | Falzone, C.J. / Vu, B.C. / Scott, N.L. / Lecomte, J.T. | ||||||
Citation | Journal: J.MOL.BIOL. / Year: 2002 Title: The Solution Structure of the Recombinant Hemoglobin from the Cyanobacterium Synechocystis sp. PCC 6803 in its Hemichrome State Authors: Falzone, C.J. / Vu, B.C. / Scott, N.L. / Lecomte, J.T. #1: Journal: J.Biomol.NMR / Year: 2002 Title: Assignment of the 1H, 13C, and 15N Signals of Synechocystis sp. PCC 6803 Methemoglobin Authors: Falzone, C.J. / Lecomte, J.T. #2: Journal: Protein Sci. / Year: 2000 Title: Cloning, Expression, Purification, and Preliminary Characterization of a Putative Hemoglobin from the Cyanobacterium Synechocystis sp. PCC 6803 Authors: Scott, N.L. / Lecomte, J.T. | ||||||
History |
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Remark 999 | SEQUENCE AUTHORS INFORMED THAT MET 1 IN SWISSPROT P73925 WAS CLEAVED POST-TRANSLATIONALLY |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1mwb.cif.gz | 856.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1mwb.ent.gz | 726 KB | Display | PDB format |
PDBx/mmJSON format | 1mwb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1mwb_validation.pdf.gz | 459.4 KB | Display | wwPDB validaton report |
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Full document | 1mwb_full_validation.pdf.gz | 582.2 KB | Display | |
Data in XML | 1mwb_validation.xml.gz | 40.7 KB | Display | |
Data in CIF | 1mwb_validation.cif.gz | 65.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mw/1mwb ftp://data.pdbj.org/pub/pdb/validation_reports/mw/1mwb | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13757.427 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Synechocystis sp. (bacteria) / Strain: PCC 6803 / Gene: slr2097 (glbN) / Plasmid: pET3c / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P73925 |
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#2: Chemical | ChemComp-HEM / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: This is a low-spin Fe(+3) complex. Standard methods as applied to dimagnetic proteins were used to determine the structure. |
-Sample preparation
Details |
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Sample conditions | Ionic strength: 50 mM / pH: 7.5 / Pressure: ambient / Temperature: 298 K | |||||||||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: distance geometry, simulated annealing, molecular dynamics, torsional angle database Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy and fewest violations | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with lowest energy and least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |