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- PDB-1mjp: METHIONINE APOREPRESSOR MUTANT (Q44K) COMPLEXED TO THE MINIMAL ME... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1mjp | ||||||
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Title | METHIONINE APOREPRESSOR MUTANT (Q44K) COMPLEXED TO THE MINIMAL MET CONSENSUS OPERATOR | ||||||
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![]() | TRANSCRIPTION/DNA / COMPLEX (TRANSCRIPTION REGULATION-DNA) / METJ / METHIONINE REPRESSOR / SHEET-HELIX-HELIX / SAM / S-ADENOSYL METHIONINE / TRANSCRIPTION-DNA COMPLEX | ||||||
Function / homology | ![]() methionine biosynthetic process / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / DNA binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Garvie, C.W. / Phillips, S.E.V. | ||||||
![]() | ![]() Title: Direct and indirect readout in mutant Met repressor-operator complexes. Authors: Garvie, C.W. / Phillips, S.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 54.7 KB | Display | ![]() |
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PDB format | ![]() | 43.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1mj2C ![]() 1mjmC ![]() 1mjoC ![]() 1mjqC ![]() 1cmaS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: DNA chain | Mass: 3035.003 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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#2: DNA chain | Mass: 2739.823 Da / Num. of mol.: 1 / Source method: obtained synthetically |
#3: Protein | Mass: 12028.607 Da / Num. of mol.: 2 / Mutation: Q44K Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.82 Å3/Da / Density % sol: 56 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7 Details: PROTEIN (10MG/ML) + DNA (4MG/ML) WAS CRYSTALLISED FROM 40% MPD, 100MM SODIUM CACODYLATE BUFFER, PH 7.0. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7 / Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Jun 1, 1997 / Details: MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 3.4→30 Å / Num. obs: 19851 / % possible obs: 99.3 % / Observed criterion σ(I): 3 / Redundancy: 4 % / Biso Wilson estimate: 9.8 Å2 / Rmerge(I) obs: 0.085 / Net I/σ(I): 11.5 |
Reflection shell | Resolution: 3.4→3.58 Å / Redundancy: 4.1 % / Rmerge(I) obs: 0.085 / Mean I/σ(I) obs: 3.3 / Rsym value: 0.231 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: DIFFERENCE FOURIER METHODS Starting model: PDB ENTRY 1CMA Resolution: 3.4→30 Å / Rfactor Rfree error: 0.014 / Data cutoff high absF: 10000000 / Data cutoff low absF: 0.001 / Isotropic thermal model: GROUP / Cross valid method: THROUGHOUT / σ(F): 0 / Details: BULK SOLVENT MODEL USED
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Displacement parameters | Biso mean: 72.8 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.4→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.4→3.48 Å / Rfactor Rfree error: 0.053 / Total num. of bins used: 15
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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