+Open data
-Basic information
Entry | Database: PDB / ID: 1cma | ||||||
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Title | MET REPRESSOR/DNA COMPLEX + S-ADENOSYL-METHIONINE | ||||||
Components |
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Keywords | TRANSCRIPTION/DNA / PROTEIN-DNA COMPLEX / DOUBLE HELIX / TRANSCRIPTION-DNA COMPLEX | ||||||
Function / homology | Function and homology information methionine biosynthetic process / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / DNA binding / cytosol Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.8 Å | ||||||
Authors | Somers, W.S. / Phillips, S.E.V. | ||||||
Citation | Journal: Nature / Year: 1992 Title: Crystal structure of the met repressor-operator complex at 2.8 A resolution reveals DNA recognition by beta-strands. Authors: Somers, W.S. / Phillips, S.E. #1: Journal: Nature / Year: 1989 Title: Three-Dimensional Crystal Structures of Escherichia Coli met Repressor with and without Corepressor Authors: Rafferty, J.B. / Somers, W.S. / Saint-Girons, I. / Phillips, S.E.V. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1cma.cif.gz | 68 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1cma.ent.gz | 49.2 KB | Display | PDB format |
PDBx/mmJSON format | 1cma.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1cma_validation.pdf.gz | 492.1 KB | Display | wwPDB validaton report |
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Full document | 1cma_full_validation.pdf.gz | 509.7 KB | Display | |
Data in XML | 1cma_validation.xml.gz | 8.8 KB | Display | |
Data in CIF | 1cma_validation.cif.gz | 12.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cm/1cma ftp://data.pdbj.org/pub/pdb/validation_reports/cm/1cma | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: DNA chain | Mass: 3035.003 Da / Num. of mol.: 1 / Source method: obtained synthetically | ||||
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#2: DNA chain | Mass: 2739.823 Da / Num. of mol.: 1 / Source method: obtained synthetically | ||||
#3: Protein | Mass: 12027.557 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A8U6 #4: Chemical | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3 Å3/Da / Density % sol: 58.99 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 7 / Details: pH 7.00, VAPOR DIFFUSION, HANGING DROP | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS pH: 7 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Ambient temp details: ROOM TEMPERATURE |
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Diffraction source | Source: SYNCHROTRON / Site: SRS / Beamline: PX9.6 |
Detector | Type: ARNDT-WONACOTT / Detector: OSCILLATION CAMERA |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Highest resolution: 2.6 Å / Num. obs: 8856 |
Reflection | *PLUS Highest resolution: 2.8 Å / % possible obs: 97.7 % / Num. measured all: 33481 / Rmerge(I) obs: 0.074 |
-Processing
Software | Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||
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Refinement | Resolution: 2.8→10 Å /
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Refinement step | Cycle: LAST / Resolution: 2.8→10 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2.8 Å / Lowest resolution: 10 Å / Rfactor obs: 0.22 | ||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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