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Yorodumi- PDB-1mfq: Crystal Structure Analysis of a Ternary S-Domain Complex of Human... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1mfq | ||||||
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Title | Crystal Structure Analysis of a Ternary S-Domain Complex of Human Signal Recognition Particle | ||||||
Components |
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Keywords | SIGNALING PROTEIN/RNA / RNA-protein complex / A-minor motif / 3-helix junction / SIGNALING PROTEIN-RNA COMPLEX | ||||||
Function / homology | Function and homology information SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition / signal recognition particle, endoplasmic reticulum targeting / granulocyte differentiation / signal recognition particle / cotranslational protein targeting to membrane / endoplasmic reticulum signal peptide binding / signal-recognition-particle GTPase / protein targeting to ER / SRP-dependent cotranslational protein targeting to membrane / 7S RNA binding ...SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition / signal recognition particle, endoplasmic reticulum targeting / granulocyte differentiation / signal recognition particle / cotranslational protein targeting to membrane / endoplasmic reticulum signal peptide binding / signal-recognition-particle GTPase / protein targeting to ER / SRP-dependent cotranslational protein targeting to membrane / 7S RNA binding / exocrine pancreas development / SRP-dependent cotranslational protein targeting to membrane, translocation / SRP-dependent cotranslational protein targeting to membrane / ribonucleoprotein complex binding / neutrophil chemotaxis / GDP binding / nuclear body / nuclear speck / GTPase activity / nucleolus / GTP binding / endoplasmic reticulum / ATP hydrolysis activity / RNA binding / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SIRAS / Resolution: 3.1 Å | ||||||
Authors | Kuglstatter, A. / Oubridge, C. / Nagai, K. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2002 Title: Induced structural changes of 7SL RNA during the assembly of human signal recognition particle Authors: Kuglstatter, A. / Oubridge, C. / Nagai, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1mfq.cif.gz | 131 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1mfq.ent.gz | 96.3 KB | Display | PDB format |
PDBx/mmJSON format | 1mfq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1mfq_validation.pdf.gz | 470.1 KB | Display | wwPDB validaton report |
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Full document | 1mfq_full_validation.pdf.gz | 490 KB | Display | |
Data in XML | 1mfq_validation.xml.gz | 16.5 KB | Display | |
Data in CIF | 1mfq_validation.cif.gz | 22.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mf/1mfq ftp://data.pdbj.org/pub/pdb/validation_reports/mf/1mfq | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-RNA chain , 1 types, 1 molecules A
#1: RNA chain | Mass: 41566.715 Da / Num. of mol.: 1 / Fragment: S-domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: in vitro transcription with T7 RNA polymerase / Plasmid: pUC18 |
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-Signal recognition particle ... , 2 types, 2 molecules BC
#2: Protein | Mass: 12561.688 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: SRP19 / Plasmid: pET23d / Production host: Escherichia coli (E. coli) / Strain (production host): JM109(DE3) / References: UniProt: P09132 |
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#3: Protein | Mass: 14733.043 Da / Num. of mol.: 1 / Fragment: M-domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: SRP54 / Plasmid: pRK172 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P61011 |
-Non-polymers , 3 types, 26 molecules
#4: Chemical | ChemComp-MG / #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.68 Å3/Da / Density % sol: 66.57 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 300.5 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 14% (w/v) PEG8000, 100mM Na-cacodylate, 400mM lithium sulfate, 80mM magnesium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300.5K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS pH: 7.4 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-4 / Wavelength: 0.9393 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Feb 19, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9393 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→50 Å / Num. obs: 19354 / % possible obs: 99.7 % / Observed criterion σ(I): -3 / Redundancy: 16.6 % / Rsym value: 0.117 / Net I/σ(I): 24.3 |
Reflection shell | Resolution: 3.1→3.21 Å / Redundancy: 15.9 % / Mean I/σ(I) obs: 6.2 / Num. unique all: 1886 / Rsym value: 0.602 / % possible all: 100 |
Reflection | *PLUS Rmerge(I) obs: 0.117 |
Reflection shell | *PLUS % possible obs: 100 % / Rmerge(I) obs: 0.602 |
-Processing
Software |
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Refinement | Method to determine structure: SIRAS Starting model: PDB entries 1L9A (RNA), 1JID (SRP19), 1QB2 (SRP54 M-domain) Resolution: 3.1→49.63 Å / Rfactor Rfree error: 0.008 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 57.551 Å2 / ksol: 0.264604 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 97.6 Å2
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Refine analyze | Luzzati coordinate error free: 0.43 Å / Luzzati sigma a free: 0.45 Å | ||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.1→49.63 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.1→3.29 Å / Rfactor Rfree error: 0.03 / Total num. of bins used: 6
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Xplor file |
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Refinement | *PLUS % reflection Rfree: 5 % / Rfactor Rfree: 0.267 / Rfactor Rwork: 0.229 | ||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.383 / Rfactor Rwork: 0.298 |