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Open data
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Basic information
Entry | Database: PDB / ID: 1mcx | ||||||
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Title | STRUCTURE OF FULL-LENGTH ANNEXIN A1 IN THE PRESENCE OF CALCIUM | ||||||
![]() | ANNEXIN I | ||||||
![]() | METAL BINDING PROTEIN / calcium/phospholipid binding protein | ||||||
Function / homology | ![]() regulation of interleukin-1 production / regulation of leukocyte migration / granulocyte chemotaxis / positive regulation of T-helper 1 cell differentiation / phospholipase A2 inhibitor activity / regulation of hormone secretion / negative regulation of T-helper 2 cell differentiation / neutrophil activation / calcium-dependent phospholipid binding / motile cilium ...regulation of interleukin-1 production / regulation of leukocyte migration / granulocyte chemotaxis / positive regulation of T-helper 1 cell differentiation / phospholipase A2 inhibitor activity / regulation of hormone secretion / negative regulation of T-helper 2 cell differentiation / neutrophil activation / calcium-dependent phospholipid binding / motile cilium / negative regulation of exocytosis / cellular response to glucocorticoid stimulus / vesicle membrane / positive regulation of wound healing / phosphatidylserine binding / monocyte chemotaxis / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / phagocytic cup / lateral plasma membrane / phagocytosis / positive regulation of T cell proliferation / positive regulation of interleukin-2 production / regulation of cell shape / early endosome membrane / regulation of inflammatory response / actin cytoskeleton organization / basolateral plasma membrane / adaptive immune response / inflammatory response / apical plasma membrane / innate immune response / calcium ion binding / signal transduction / extracellular space / extracellular exosome / nucleus / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Luecke, H. / Rosengarth, A. | ||||||
![]() | ![]() Title: A Calcium-driven Conformational Switch of the N-terminaland Core Domains of Annexin A1 Authors: Luecke, H. / Rosengarth, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 80.6 KB | Display | ![]() |
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PDB format | ![]() | 62.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1hm6S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 38802.355 Da / Num. of mol.: 1 / Mutation: S51L, H69L, L231P, N289I Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||
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#2: Chemical | ChemComp-CA / #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.6 Å3/Da / Density % sol: 65.84 % | ||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 8 Details: PEG 1000, imidazol, calciumacetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K | ||||||||||||||||||||||||
Crystal grow | *PLUS Method: unknown | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Mar 26, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.03→60 Å / Num. obs: 37069 / Observed criterion σ(I): -3 / Redundancy: 15.3 % / Rmerge(I) obs: 0.117 / Net I/σ(I): 27.2 |
Reflection | *PLUS Lowest resolution: 60 Å / % possible obs: 97.9 % / Num. measured all: 568454 |
Reflection shell | *PLUS Highest resolution: 2.03 Å / Lowest resolution: 2.07 Å / % possible obs: 95.7 % / Rmerge(I) obs: 0.891 / Mean I/σ(I) obs: 3.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1HM6 Resolution: 2.03→60 Å / Isotropic thermal model: isotropic / Cross valid method: Rfree / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 26.7 Å2 | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.03→60 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 60 Å / Rfactor Rfree: 0.2311 / Rfactor Rwork: 0.1962 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_angle_deg / Dev ideal: 1.061 |