+Open data
-Basic information
Entry | Database: PDB / ID: 1hm6 | ||||||
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Title | X-RAY STRUCTURE OF FULL-LENGTH ANNEXIN 1 | ||||||
Components | ANNEXIN 1 | ||||||
Keywords | METAL / LIPID BINDING PROTEIN / phospholipid/Ca(2+)-binding protein / calcium-free form / full-length protein comprising protein core and N-terminal domain | ||||||
Function / homology | Function and homology information regulation of interleukin-1 production / prolactin secretion / myoblast migration involved in skeletal muscle regeneration / regulation of leukocyte migration / granulocyte chemotaxis / positive regulation of T-helper 1 cell differentiation / phospholipase A2 inhibitor activity / regulation of hormone secretion / positive regulation of vesicle fusion / neutrophil clearance ...regulation of interleukin-1 production / prolactin secretion / myoblast migration involved in skeletal muscle regeneration / regulation of leukocyte migration / granulocyte chemotaxis / positive regulation of T-helper 1 cell differentiation / phospholipase A2 inhibitor activity / regulation of hormone secretion / positive regulation of vesicle fusion / neutrophil clearance / prostate gland development / negative regulation of T-helper 2 cell differentiation / positive regulation of neutrophil apoptotic process / endocrine pancreas development / positive regulation of prostaglandin biosynthetic process / cadherin binding involved in cell-cell adhesion / hepatocyte differentiation / neutrophil activation / calcium-dependent phospholipid binding / gliogenesis / negative regulation of exocytosis / motile cilium / cellular response to glucocorticoid stimulus / insulin secretion / alpha-beta T cell differentiation / DNA duplex unwinding / arachidonic acid secretion / positive regulation of cell migration involved in sprouting angiogenesis / positive regulation of wound healing / phosphatidylserine binding / monocyte chemotaxis / phagocytic cup / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / response to X-ray / lateral plasma membrane / estrous cycle / cellular response to vascular endothelial growth factor stimulus / positive regulation of G1/S transition of mitotic cell cycle / phagocytosis / positive regulation of T cell proliferation / keratinocyte differentiation / positive regulation of interleukin-2 production / response to interleukin-1 / response to peptide hormone / cellular response to hydrogen peroxide / response to estradiol / regulation of cell shape / early endosome membrane / regulation of inflammatory response / actin cytoskeleton organization / basolateral plasma membrane / adaptive immune response / cell surface receptor signaling pathway / inflammatory response / apical plasma membrane / innate immune response / calcium ion binding / signal transduction / extracellular space / extracellular exosome / nucleus / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Sus scrofa (pig) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIR, MOLECULAR REPLACEMENT / Resolution: 1.8 Å | ||||||
Authors | Rosengarth, A. / Gerke, V. / Luecke, H. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2001 Title: X-ray structure of full-length annexin 1 and implications for membrane aggregation. Authors: Rosengarth, A. / Gerke, V. / Luecke, H. #1: Journal: Acta Crystallogr.,Sect.D / Year: 2000 Title: Crystallization and Preliminary X-ray Analysis of Full-length Annexin I Comprising the Core and N-terminal Domain. Authors: Rosengarth, A. / Luecke, H. | ||||||
History |
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Remark 300 | BIOMOLECULE: 1 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT WHICH CONSISTS OF 2 CHAIN(S) ...BIOMOLECULE: 1 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT WHICH CONSISTS OF 2 CHAIN(S). THE BIOLOGICAL MOLECULE MAY BE A MONOMER OR HIGHER OLIGOMER. | ||||||
Remark 999 | SEQUENCE Seemann et al. deposited the cDNA sequence in genbank that they obtained from a library ... SEQUENCE Seemann et al. deposited the cDNA sequence in genbank that they obtained from a library screening. However, they were not able to get the complete cDNA. Later on, they introduced the naturally occuring (but missing) 5 amino acid residues at the N-terminal domain by PCR. This is also described in their paper (Seemann et al. (1996), Mol. Biol. Cell 7, 1359-1374). According to Seemann et al. (1996), the sequence shown herein starts with residue Met1, that would be residue -5 according to the truncated genbank entry, although there was no electron density observed for this residue. The amino acid sequence does not exactly match the (DNA) sequence deposited in the genbank by Seemann et al. However, the authors received the original construct from them and the amino acid sequence is according to his paper (Seemann et al., (1996), see above) (confirmed by amino acid sequencing). The electron densities of the respective amino acid residues in the annealed omit map showed clearly that the following changes have to be made in comparison to his genbank sequence: 1) residue 51 is a Leu (not Ser) 2) residue 69 is a Leu (not His) 3) residue 231 is a Pro (not Leu) 4) residue 289 is a Ile (not Asn) |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1hm6.cif.gz | 163.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1hm6.ent.gz | 127.8 KB | Display | PDB format |
PDBx/mmJSON format | 1hm6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1hm6_validation.pdf.gz | 390 KB | Display | wwPDB validaton report |
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Full document | 1hm6_full_validation.pdf.gz | 398.5 KB | Display | |
Data in XML | 1hm6_validation.xml.gz | 14.9 KB | Display | |
Data in CIF | 1hm6_validation.cif.gz | 26.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hm/1hm6 ftp://data.pdbj.org/pub/pdb/validation_reports/hm/1hm6 | HTTPS FTP |
-Related structure data
Related structure data | 1ainS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 38802.355 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sus scrofa (pig) / Gene: ANX1 / Plasmid: PKKANX1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)PLYSS / References: UniProt: P19619 #2: Chemical | ChemComp-SO4 / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.52 Å3/Da / Density % sol: 51.08 % | ||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 2.2 M Ammonium sulfate, 0.1 M Tris/HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K | ||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 5.0.2 / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Oct 28, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→40 Å / Num. all: 68225 / Num. obs: 68225 / % possible obs: 93 % / Redundancy: 10 % / Rmerge(I) obs: 0.082 / Net I/σ(I): 14.6 |
Reflection shell | Resolution: 1.8→1.83 Å / Rmerge(I) obs: 0.599 / Mean I/σ(I) obs: 2.1 / Num. unique all: 3596 / % possible all: 99.4 |
Reflection | *PLUS Num. measured all: 679717 |
Reflection shell | *PLUS % possible obs: 99.4 % |
-Processing
Software |
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Refinement | Method to determine structure: MIR, MOLECULAR REPLACEMENT Starting model: PDB entry 1AIN Resolution: 1.8→40 Å / Cross valid method: RFREE / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 26.62 Å2 | ||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→40 Å
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Refine LS restraints |
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Xplor file |
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | ||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 40 Å / σ(F): 0 / % reflection Rfree: 5 % / Rfactor all: 0.223 | ||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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