+Open data
-Basic information
Entry | Database: PDB / ID: 1aii | |||||||||
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Title | ANNEXIN III | |||||||||
Components | ANNEXIN III | |||||||||
Keywords | CALCIUM/PHOSPHOLIPID BINDING PROTEIN / ANNEXIN / PHOSPHOLIPASE A2 INHIBITOR / CALCIUM-PHOSPHOLIPID BINDING PROTEIN complex | |||||||||
Function / homology | Function and homology information phospholipase A2 inhibitor activity / neutrophil degranulation / positive regulation of DNA metabolic process / specific granule / response to growth factor / calcium-dependent phospholipid binding / animal organ regeneration / phagocytosis / response to glucocorticoid / positive regulation of endothelial cell migration ...phospholipase A2 inhibitor activity / neutrophil degranulation / positive regulation of DNA metabolic process / specific granule / response to growth factor / calcium-dependent phospholipid binding / animal organ regeneration / phagocytosis / response to glucocorticoid / positive regulation of endothelial cell migration / hippocampus development / phagocytic vesicle membrane / calcium-dependent protein binding / positive regulation of angiogenesis / positive regulation of DNA-binding transcription factor activity / defense response to bacterium / axon / dendrite / neuronal cell body / calcium ion binding / extracellular exosome / membrane / plasma membrane / cytoplasm Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.95 Å | |||||||||
Authors | Lewit-Bentley, A. / Perron, B. | |||||||||
Citation | Journal: J.Biol.Chem. / Year: 1997 Title: Can enzymatic activity, or otherwise, be inferred from structural studies of annexin III? Authors: Perron, B. / Lewit-Bentley, A. / Geny, B. / Russo-Marie, F. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1aii.cif.gz | 83.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1aii.ent.gz | 61.1 KB | Display | PDB format |
PDBx/mmJSON format | 1aii.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ai/1aii ftp://data.pdbj.org/pub/pdb/validation_reports/ai/1aii | HTTPS FTP |
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-Related structure data
Related structure data | 1axnS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 36427.270 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) Description: PLASMID SOURCE PUC (BIOGEN, CAMBRIDGE, MA, USA) Plasmid: PGEX-2T / Production host: Escherichia coli (E. coli) / Strain (production host): NB42 / References: UniProt: P12429 | ||||||
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#2: Chemical | ChemComp-CA / #3: Chemical | ChemComp-SO4 / | #4: Chemical | ChemComp-ETA / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 38.87 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.8 Details: 18MG/ML PROTEIN, 10MM CACL2 IN 50 MM HEPES AT PH=7.8 WAS EQUILIBRATED AGAINST 50% AMMONIUM SULFATE IN THE SAME BUFFER. | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.5 / Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 300 K |
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Diffraction source | Source: SYNCHROTRON / Site: LURE / Beamline: D41A / Wavelength: 1.38 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jun 8, 1995 |
Radiation | Monochromator: GE(111) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.38 Å / Relative weight: 1 |
Reflection | Resolution: 1.95→18.95 Å / Num. obs: 20818 / % possible obs: 98.5 % / Observed criterion σ(I): 0 / Redundancy: 2.9 % / Biso Wilson estimate: 0.6 Å2 / Rsym value: 0.072 / Net I/σ(I): 4.71 |
Reflection shell | Resolution: 1.95→2 Å / Redundancy: 2.08 % / Mean I/σ(I) obs: 4.7 / Rsym value: 0.139 / % possible all: 83.5 |
Reflection | *PLUS Num. measured all: 78671 / Rmerge(I) obs: 0.072 |
Reflection shell | *PLUS % possible obs: 83.5 % / Rmerge(I) obs: 0.139 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1AXN Resolution: 1.95→20 Å / Cross valid method: THROUGHOUT / σ(F): 1
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Displacement parameters | Biso mean: 18.8 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.95→20 Å
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Refine LS restraints |
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Software | *PLUS Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.193 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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