Mass: 7983.740 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: This sequence occurs naturally in complementary RNA of the influenza A virus genome segments.
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
2
2D NOESY
2
2
1
2D NOESY
2
3
1
2D TOCSY
2
4
1
DQF-COSY
2
5
3
2D NOESY
2
6
4
2D NOESY
2
7
1
HETCOR
2
8
1
3D 13C NOESY-HSQC
2
9
1
3D (H)CCH-COSY
2
10
1
3D (H)CCH-COSY-TOCSY
NMR details
Text: The structure was determined using 3D heteronuclear nmr spectroscopy.
1mM influenza A virus cRNA promoter (25mer) U-15N, 13C; 20mM phosphate buffer(pH 6.0)0.1mM EDTA
100% D2O
Sample conditions
Conditions-ID
Ionic strength
pH
Pressure (kPa)
Temperature (K)
1
20mMNaPi0.1mMEDTA
6
ambient
300K
2
20mMNaPi0.1mMEDTA
6
ambient
280K
3
20mMNaPi0.1mMEDTA
6
ambient
290K
4
20mMNaPi0.1mMEDTA
6
ambient
310K
Crystal grow
*PLUS
Method: other / Details: NMR
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NMR measurement
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelength
Relative weight: 1
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Bruker DMX
Bruker
DMX
600
1
Bruker DMX
Bruker
DMX
800
2
Bruker AMX
Bruker
AMX
500
3
Varian INOVA
Varian
INOVA
600
4
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Processing
NMR software
Name
Version
Developer
Classification
Felix
95
Biosym,inc.
processing
VNMR
6.1c
Varian, inc.
collection
CNS
1
Brunger
structuresolution
CNS
1
Brunger
refinement
Refinement
Method: torsion angle dynamics / Software ordinal: 1 Details: The structures are based on a total of 834 restraints, 592 are NOE-derived distance restraints, 140 torsion angle restraints, 54 planarity restraints and 48 base-pair restraints.
NMR representative
Selection criteria: lowest energy
NMR ensemble
Conformer selection criteria: structures with the least restraint violations,structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 31
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