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- PDB-1m45: CRYSTAL STRUCTURE OF MLC1P BOUND TO IQ2 OF MYO2P, A CLASS V MYOSIN -
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Open data
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Basic information
Entry | Database: PDB / ID: 1m45 | ||||||
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Title | CRYSTAL STRUCTURE OF MLC1P BOUND TO IQ2 OF MYO2P, A CLASS V MYOSIN | ||||||
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![]() | CELL CYCLE PROTEIN / Protein-Peptide complex / IQ motif / myosin light chain | ||||||
Function / homology | ![]() MIH complex / regulation of cell wall organization or biogenesis / regulation of actomyosin contractile ring contraction / RHO GTPases activate PAKs / peroxisome inheritance / protein localization to cell division site involved in mitotic actomyosin contractile ring assembly / myosin II heavy chain binding / RHOT2 GTPase cycle / RHOT1 GTPase cycle / Myo2p-Vac17p-Vac8p transport complex ...MIH complex / regulation of cell wall organization or biogenesis / regulation of actomyosin contractile ring contraction / RHO GTPases activate PAKs / peroxisome inheritance / protein localization to cell division site involved in mitotic actomyosin contractile ring assembly / myosin II heavy chain binding / RHOT2 GTPase cycle / RHOT1 GTPase cycle / Myo2p-Vac17p-Vac8p transport complex / membrane addition at site of cytokinesis / RHOU GTPase cycle / cellular bud neck contractile ring / vesicle targeting / meiotic nuclear membrane microtubule tethering complex / site of polarized growth / myosin V complex / mitotic actomyosin contractile ring assembly / vacuole inheritance / Golgi inheritance / incipient cellular bud site / cellular bud tip / septum digestion after cytokinesis / myosin V binding / cellular bud neck / mating projection tip / vesicle transport along actin filament / fungal-type vacuole membrane / vesicle docking involved in exocytosis / myosin II complex / microfilament motor activity / filamentous actin / actin filament bundle / establishment of mitotic spindle orientation / vesicle-mediated transport / transport vesicle / regulation of cytokinesis / actin filament organization / actin filament binding / protein transport / actin cytoskeleton / vesicle / calmodulin binding / calcium ion binding / ATP binding / identical protein binding / membrane / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Terrak, M. / Dominguez, R. | ||||||
![]() | ![]() Title: Two distinct myosin light chain structures are induced by specific variations within the bound IQ motifs-functional implications Authors: Terrak, M. / Wu, G. / Stafford, W.F. / Lu, R.C. / Dominguez, R. #1: ![]() Title: Crystallisation, X-ray characterization and selenomethionine phasing of Mlc1p bound to IQ motifs from myosin V Authors: Terrak, M. / Otterbein, L.R. / Wu, G. / Palecanda, L.A. / Lu, R.C. / Dominguez, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 51.9 KB | Display | ![]() |
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PDB format | ![]() | 37.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 365.7 KB | Display | ![]() |
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Full document | ![]() | 368.3 KB | Display | |
Data in XML | ![]() | 5 KB | Display | |
Data in CIF | ![]() | 8.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16332.213 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: MLC1 / Plasmid: pAED4 / Species (production host): Escherichia coli / Production host: ![]() ![]() |
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#2: Protein/peptide | Mass: 2993.420 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: The peptide was chemically synthesized. The sequence of the peptide is naturally found in Saccharomyces cerevisiae (Baker's yeast). References: UniProt: P19524 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.81 Å3/Da / Density % sol: 32.13 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: PEG 3350, sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jun 1, 2000 |
Radiation | Monochromator: Si(111) double-crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.64→25.4 Å / Num. all: 17258 / Num. obs: 17258 / % possible obs: 97.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 10.3 % / Rmerge(I) obs: 0.036 / Net I/σ(I): 25.6 |
Reflection shell | Resolution: 1.64→1.71 Å / Rmerge(I) obs: 0.2 / Mean I/σ(I) obs: 6.1 / % possible all: 86 |
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Processing
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Refinement | Method to determine structure: ![]() Resolution: 1.65→25 Å / Cor.coef. Fo:Fc: 0.952 / Cor.coef. Fo:Fc free: 0.936 / SU B: 2.229 / SU ML: 0.076 / Isotropic thermal model: overall anisotropic / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.131 / ESU R Free: 0.121 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: CNS 1.0 was also used at the beginning of the refinement of this structure
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 24.235 Å2
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Refinement step | Cycle: LAST / Resolution: 1.65→25 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.65→1.693 Å / Total num. of bins used: 20
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