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Yorodumi- PDB-1m02: NMR Structure of PW2 Bound to SDS Micelles: A Tryptophan-rich Ant... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1m02 | ||||||
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Title | NMR Structure of PW2 Bound to SDS Micelles: A Tryptophan-rich Anticocidial Peptide Selected from Phage Display Libraries | ||||||
Components | HIS-PRO-LEU-LYS-GLN-TYR-TRP-TRP-ARG-PRO-SER-ILE | ||||||
Keywords | DE NOVO PROTEIN / Anticoccidial peptide / Eimeria / SDS / micelle / antimicrobial / pw2 | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Tinoco, L.W. / da Silva Jr., A. / Leite, A. / Valente, A.P. / Almeida, F.C. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2002 Title: NMR structure of PW2 bound to SDS micelles. A tryptophan-rich anticoccidial peptide selected from phage display libraries Authors: Tinoco, L.W. / Da Silva Jr., A. / Leite, A. / Valente, A.P. / Almeida, F.C. #1: Journal: MOL.BIOCHEM.PARASITOL. / Year: 2002 Title: Avian anticoccidial activity of a novel membrane-interactive peptide selected from phage display libraries Authors: da Silva Jr., A. / Kawazoe, U. / Freitas, F.F. / Gatti, M.S. / Dolder, H. / Schumacher, R.I. / Juliano, M.A. / da Silva, M.J. / Leite, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1m02.cif.gz | 77.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1m02.ent.gz | 55.5 KB | Display | PDB format |
PDBx/mmJSON format | 1m02.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1m02_validation.pdf.gz | 335.8 KB | Display | wwPDB validaton report |
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Full document | 1m02_full_validation.pdf.gz | 426.5 KB | Display | |
Data in XML | 1m02_validation.xml.gz | 6.2 KB | Display | |
Data in CIF | 1m02_validation.cif.gz | 9.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m0/1m02 ftp://data.pdbj.org/pub/pdb/validation_reports/m0/1m02 | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 1613.881 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: solid phase synthesis |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 4 mM PW2 Solvent system: 20 mM sodium phosphate buffer, 160 mM SDS-d25, 10% D20 |
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Sample conditions | Ionic strength: 100 mM sodium chloride / pH: 5.0 / Pressure: ambient / Temperature: 298 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 / Details: cartesian dynamics | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 19 |