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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1lph | ||||||
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タイトル | LYS(B28)PRO(B29)-HUMAN INSULIN | ||||||
![]() | (INSULIN) x 2 | ||||||
![]() | HORMONE / INSULIN ANALOGUE / GLUCOSE METABOLISM | ||||||
機能・相同性 | ![]() negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / positive regulation of nitric oxide mediated signal transduction / negative regulation of fatty acid metabolic process / Signaling by Insulin receptor / negative regulation of feeding behavior / IRS activation / Insulin processing / regulation of protein secretion / positive regulation of peptide hormone secretion ...negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / positive regulation of nitric oxide mediated signal transduction / negative regulation of fatty acid metabolic process / Signaling by Insulin receptor / negative regulation of feeding behavior / IRS activation / Insulin processing / regulation of protein secretion / positive regulation of peptide hormone secretion / positive regulation of respiratory burst / Regulation of gene expression in beta cells / negative regulation of acute inflammatory response / positive regulation of protein autophosphorylation / alpha-beta T cell activation / positive regulation of dendritic spine maintenance / Synthesis, secretion, and deacylation of Ghrelin / negative regulation of respiratory burst involved in inflammatory response / negative regulation of protein secretion / positive regulation of glycogen biosynthetic process / negative regulation of gluconeogenesis / Signal attenuation / fatty acid homeostasis / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / positive regulation of insulin receptor signaling pathway / negative regulation of lipid catabolic process / regulation of protein localization to plasma membrane / positive regulation of lipid biosynthetic process / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / activation of protein kinase B activity / COPI-mediated anterograde transport / transport vesicle / nitric oxide-cGMP-mediated signaling / negative regulation of reactive oxygen species biosynthetic process / Insulin receptor recycling / insulin-like growth factor receptor binding / positive regulation of brown fat cell differentiation / NPAS4 regulates expression of target genes / neuron projection maintenance / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of nitric-oxide synthase activity / positive regulation of mitotic nuclear division / Insulin receptor signalling cascade / regulation of transmembrane transporter activity / positive regulation of glycolytic process / positive regulation of long-term synaptic potentiation / endosome lumen / positive regulation of cytokine production / acute-phase response / positive regulation of D-glucose import / positive regulation of protein secretion / positive regulation of cell differentiation / Regulation of insulin secretion / insulin receptor binding / wound healing / negative regulation of protein catabolic process / hormone activity / regulation of synaptic plasticity / positive regulation of neuron projection development / positive regulation of protein localization to nucleus / Golgi lumen / cognition / glucose metabolic process / vasodilation / insulin receptor signaling pathway / glucose homeostasis / cell-cell signaling / regulation of protein localization / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / protease binding / positive regulation of cell growth / secretory granule lumen / positive regulation of canonical NF-kappaB signal transduction / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of MAPK cascade / positive regulation of cell migration / G protein-coupled receptor signaling pathway / Amyloid fiber formation / endoplasmic reticulum lumen / Golgi membrane / negative regulation of gene expression / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of DNA-templated transcription / extracellular space / extracellular region / identical protein binding 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() | ||||||
![]() | Ciszak, E. / Beals, J.M. / Frank, B.H. / Baker, J.C. / Carter, N.D. / Smith, G.D. | ||||||
![]() | ![]() タイトル: Role of C-terminal B-chain residues in insulin assembly: the structure of hexameric LysB28ProB29-human insulin. 著者: Ciszak, E. / Beals, J.M. / Frank, B.H. / Baker, J.C. / Carter, N.D. / Smith, G.D. #1: ![]() タイトル: The Structure of a Complex of Hexameric Insulin and 4'-Hydroxyacetanilide 著者: Smith, G.D. / Ciszak, E. #2: ![]() タイトル: Crystallographic Evidence for Dual Coordination Around Zinc in the T3R3 Human Insulin Hexamer 著者: Ciszak, E. / Smith, G.D. #3: ![]() タイトル: Structural Stability in the 4-Zinc Human Insulin Hexamer 著者: Smith, G.D. / Swenson, D.C. / Dodson, E.J. / Dodson, G.G. / Reynolds, C.D. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 35.3 KB | 表示 | ![]() |
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PDB形式 | ![]() | 24 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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Components on special symmetry positions |
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要素
-タンパク質・ペプチド , 2種, 4分子 ACBD
#1: タンパク質・ペプチド | 分子量: 2383.698 Da / 分子数: 2 / 変異: CHAIN B, D, P28K, K29P / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() #2: タンパク質・ペプチド | 分子量: 3433.953 Da / 分子数: 2 / 変異: CHAIN B, D, P28K, K29P / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() |
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-非ポリマー , 4種, 63分子 






#3: 化合物 | #4: 化合物 | ChemComp-IPH / | #5: 化合物 | ChemComp-CL / | #6: 水 | ChemComp-HOH / | |
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-詳細
Has protein modification | Y |
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非ポリマーの詳細 | EACH OF TWO ZINC IONS IS COORDINATED BY THE THREE SYMMETRY RELATED HIS B 10 SIDE CHAINS. THE ...EACH OF TWO ZINC IONS IS COORDINATE |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.21 Å3/Da / 溶媒含有率: 33 % | ||||||||||||||||||||
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結晶 | *PLUS | ||||||||||||||||||||
結晶化 | *PLUS pH: 5.9 / 手法: unknown | ||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
検出器 | タイプ: RIGAKU RAXIS IIC / 検出器: IMAGE PLATE / 日付: 1994年6月9日 |
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放射 | 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 相対比: 1 |
反射 | 解像度: 2.3→38.5 Å / Num. obs: 3910 / % possible obs: 83.4 % / Observed criterion σ(I): 0 / 冗長度: 5 % / Rmerge(I) obs: 0.06 |
反射 | *PLUS Num. measured all: 19299 / Rmerge(I) obs: 0.06 |
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解析
ソフトウェア |
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精密化 | 解像度: 2.3→8 Å / σ(F): 2 /
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原子変位パラメータ | Biso mean: 37.3 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 2.3→8 Å
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拘束条件 |
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拘束条件 | *PLUS
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