+Open data
-Basic information
Entry | Database: PDB / ID: 1lp1 | ||||||
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Title | Protein Z in complex with an in vitro selected affibody | ||||||
Components |
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Keywords | IMMUNE SYSTEM / in vitro evolved / protein-protein complex / three-helix bundle / affibody | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Staphylococcus aureus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å | ||||||
Authors | Hogbom, M. / Eklund, M. / Nygren, P.A. / Nordlund, P. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2003 Title: Structural basis for recognition by an in vitro evolved affibody. Authors: Hogbom, M. / Eklund, M. / Nygren, P.A. / Nordlund, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1lp1.cif.gz | 39.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1lp1.ent.gz | 27.4 KB | Display | PDB format |
PDBx/mmJSON format | 1lp1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lp/1lp1 ftp://data.pdbj.org/pub/pdb/validation_reports/lp/1lp1 | HTTPS FTP |
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-Related structure data
Related structure data | 1deeS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 6447.263 Da / Num. of mol.: 1 / Fragment: In vitro selected binding protein Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (bacteria) / Production host: Escherichia coli (E. coli) | ||||
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#2: Antibody | Mass: 6648.316 Da / Num. of mol.: 1 / Fragment: RESIDUES 2-58 / Mutation: A1V, G29A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: P38507 | ||||
#3: Chemical | ChemComp-SO4 / #4: Chemical | ChemComp-MG / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 46.45 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: MgSO4, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.5 | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: MAX II / Beamline: I711 / Wavelength: 1.098 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Oct 1, 2001 |
Radiation | Monochromator: asymmetrically cut Si(111) monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.098 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→30 Å / Num. all: 6899 / Num. obs: 6899 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 8.3 % / Biso Wilson estimate: 51.3 Å2 / Rmerge(I) obs: 0.05 |
Reflection shell | Resolution: 2.3→2.38 Å / Rmerge(I) obs: 0.296 / % possible all: 99.8 |
Reflection | *PLUS Lowest resolution: 20 Å / Num. obs: 6847 / % possible obs: 99.7 % / Num. measured all: 57198 / Rmerge(I) obs: 0.05 |
Reflection shell | *PLUS % possible obs: 99.8 % / Rmerge(I) obs: 0.29 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: Polyserine model of PDB entry 1DEE, chain G Resolution: 2.3→20 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.3→20 Å
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Refine LS restraints |
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Refinement | *PLUS % reflection Rfree: 5 % / Rfactor Rfree: 0.255 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_angle_deg / Dev ideal: 1.17 |