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Yorodumi- PDB-1l2g: Structure of a C-terminally truncated form of glycoprotein D from... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1l2g | ||||||
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Title | Structure of a C-terminally truncated form of glycoprotein D from HSV-1 | ||||||
Components | Glycoprotein D | ||||||
Keywords | VIRAL PROTEIN / Ig fold / viral envelope glycoprotein | ||||||
Function / homology | Function and homology information host cell Golgi apparatus / entry receptor-mediated virion attachment to host cell / symbiont entry into host cell / viral envelope / virion membrane / membrane / metal ion binding Similarity search - Function | ||||||
Biological species | Human herpesvirus 1 (Herpes simplex virus type 1) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.85 Å | ||||||
Authors | Carfi, A. / Willis, S.H. / Whitbeck, J.C. / Krummenacher, C. / Cohen, G.H. / Eisenberg, R.J. / Wiley, D.C. | ||||||
Citation | Journal: Mol.Cell / Year: 2001 Title: Herpes simplex virus glycoprotein D bound to the human receptor HveA. Authors: Carfi, A. / Willis, S.H. / Whitbeck, J.C. / Krummenacher, C. / Cohen, G.H. / Eisenberg, R.J. / Wiley, D.C. #1: Journal: Cell(Cambridge,Mass.) / Year: 1996 Title: HERPES SIMPLEX VIRUS-1 ENTRY INTO CELLS MEDIATED BY A NOVEL MEMBER OF THE TNF/NGF RECEPTOR FAMILY Authors: MONTGOMERY, R.I. / WARNER, M.S. / LUM, B.J. / SPEAR, P.G. | ||||||
History |
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Remark 300 | BIOMOLECULE: 1, 2, 3, 4 ACCORDING TO THE AUTHOR, THIS C-TERMINALLY TRUNCATED GD MOLECULE (RESIDUES ...BIOMOLECULE: 1, 2, 3, 4 ACCORDING TO THE AUTHOR, THIS C-TERMINALLY TRUNCATED GD MOLECULE (RESIDUES 1 TO 285) IS MONOMERIC IN SOLUTION, BUT FORMED DIMERS IN THE CRYSTAL. THIS ENTRY CONTAINS TWO DIMERS, AN AB DIMER CONSISTING OF CHAINS A AND B, AND A CD DIMER CONSISTING OF CHAINS C AND D. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1l2g.cif.gz | 375.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1l2g.ent.gz | 312.1 KB | Display | PDB format |
PDBx/mmJSON format | 1l2g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l2/1l2g ftp://data.pdbj.org/pub/pdb/validation_reports/l2/1l2g | HTTPS FTP |
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-Related structure data
Related structure data | 1jmaSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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4 |
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Unit cell |
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Number of models | 2 |
-Components
#1: Protein | Mass: 31837.168 Da / Num. of mol.: 4 / Fragment: Ectodomain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Human herpesvirus 1 (Herpes simplex virus type 1) Genus: Simplexvirus / Plasmid: PFASTBAC-DUAL / Production host: Spodoptera frugiperda (fall armyworm) / Strain (production host): Sf9 / References: UniProt: P57083 #2: Sugar | ChemComp-NAG / |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.82 Å3/Da / Density % sol: 56.38 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 9 Details: Ammonium Sulfate, pH 9, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X25 / Wavelength: 1.1 Å |
Detector | Type: BRANDEIS - B4 / Detector: CCD / Date: Oct 10, 1999 |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 2.85→30 Å / Num. obs: 29319 / % possible obs: 87.1 % / Observed criterion σ(F): -2 / Observed criterion σ(I): -3 / Redundancy: 2.5 % / Biso Wilson estimate: 51 Å2 / Rsym value: 0.076 / Net I/σ(I): 11 |
Reflection shell | Resolution: 2.85→2.95 Å / Mean I/σ(I) obs: 2.7 / Rsym value: 0.23 / % possible all: 67.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1JMA Resolution: 2.85→30 Å / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: Engh & Huber Details: THE CRYSTALS ARE MEROHEDRALLY TWINNED. THE TWINNING OPERATION IS A 2 FOLD ROTATION PARALLEL TO THE A AXIS. THE TWO BLOCKS ARE REPRESENTED IN THIS ENTRY BY TWO MODELS (MODEL 1 AND MODEL 2) ...Details: THE CRYSTALS ARE MEROHEDRALLY TWINNED. THE TWINNING OPERATION IS A 2 FOLD ROTATION PARALLEL TO THE A AXIS. THE TWO BLOCKS ARE REPRESENTED IN THIS ENTRY BY TWO MODELS (MODEL 1 AND MODEL 2) CONTAINING 4 CHAINS (ABCD) EACH. MOLECULES ABCD IN MODEL 1 ARE RELATED BY A 2 FOLD ROTATION AXIS (TWIN OPERATION) TO MOLECULES ABCD OF MODEL 2. NO DETWINNING OF THE DATA WAS ATTEMPTED.
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.85→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.85→2.98 Å
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