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Open data
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Basic information
Entry | Database: PDB / ID: 1l0h | ||||||
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Title | CRYSTAL STRUCTURE OF BUTYRYL-ACP FROM E.COLI | ||||||
![]() | ACYL CARRIER PROTEIN | ||||||
![]() | LIPID TRANSPORT / acyl carrier protein / acyl chain binding / fatty acid biosynthesis | ||||||
Function / homology | ![]() lipid biosynthetic process / lipid A biosynthetic process / phosphopantetheine binding / acyl binding / acyl carrier activity / fatty acid biosynthetic process / response to xenobiotic stimulus / lipid binding / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Roujeinikova, A. / Baldock, C. / Simon, W.J. / Gilroy, J. / Baker, P.J. / Stuitje, A.R. / Rice, D.W. / Slabas, A.R. / Rafferty, J.B. | ||||||
![]() | ![]() Title: X-ray crystallographic studies on butyryl-ACP reveal flexibility of the structure around a putative acyl chain binding site Authors: Roujeinikova, A. / Baldock, C. / Simon, W.J. / Gilroy, J. / Baker, P.J. / Stuitje, A.R. / Rice, D.W. / Slabas, A.R. / Rafferty, J.B. #1: ![]() Title: Crystallisation and preliminary X-ray crystallographic studies on acyl-(acyl carrier protein) from Escherichia coli Authors: Roujeinikova, A. / Baldock, C. / Simon, W.J. / Gilroy, J. / Baker, P.J. / Stuitje, A.R. / Rice, D.W. / Rafferty, J.B. / Slabas, A.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 28.5 KB | Display | ![]() |
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PDB format | ![]() | 18.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1l0iSC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 8645.460 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.46 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 6 Details: PEG 20000, zinc chloride, sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Details: Roujeinikova, A., (2002) Acta Crystallogr., Sect.D, 58, 330. | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. obs: 10616 / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.06 |
Reflection shell | Resolution: 2→2.05 Å / Rmerge(I) obs: 0.269 |
Reflection | *PLUS Num. obs: 5485 / % possible obs: 94 % / Num. measured all: 29701 / Rmerge(I) obs: 0.06 |
Reflection shell | *PLUS % possible obs: 90 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: ACP I62M mutant, PDB ENTRY 1L0I Resolution: 2→10 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0
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Displacement parameters | Biso mean: 36 Å2 | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→10 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 10 Å / Num. reflection obs: 9273 / % reflection Rfree: 5 % | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |