+Open data
-Basic information
Entry | Database: PDB / ID: 1k0l | ||||||
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Title | Pseudomonas aeruginosa phbh R220Q free of p-OHB | ||||||
Components | P-HYDROXYBENZOATE HYDROXYLASE | ||||||
Keywords | HYDROLASE / phbh / FAD | ||||||
Function / homology | Function and homology information 4-hydroxybenzoate 3-monooxygenase (NADPH) activity / 4-hydroxybenzoate 3-monooxygenase / 4-hydroxybenzoate 3-monooxygenase activity / benzoate catabolic process via hydroxylation / FAD binding / flavin adenine dinucleotide binding / oxidoreductase activity Similarity search - Function | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Wang, J. / Ortiz-Maldonado, M. / Entsch, B. / Ballou, D. / Gatti, D.L. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2002 Title: Protein and ligand dynamics in 4-hydroxybenzoate hydroxylase. Authors: Wang, J. / Ortiz-Maldonado, M. / Entsch, B. / Massey, V. / Ballou, D. / Gatti, D.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1k0l.cif.gz | 98.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1k0l.ent.gz | 74.3 KB | Display | PDB format |
PDBx/mmJSON format | 1k0l.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1k0l_validation.pdf.gz | 490 KB | Display | wwPDB validaton report |
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Full document | 1k0l_full_validation.pdf.gz | 495.8 KB | Display | |
Data in XML | 1k0l_validation.xml.gz | 10.5 KB | Display | |
Data in CIF | 1k0l_validation.cif.gz | 16.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/k0/1k0l ftp://data.pdbj.org/pub/pdb/validation_reports/k0/1k0l | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Components on special symmetry positions |
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Details | The enzyme is a dimer in solution. But only one monomer is present in the asymmetric unit. |
-Components
#1: Protein | Mass: 44353.484 Da / Num. of mol.: 1 / Mutation: R220Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Plasmid: bl21 / Production host: Escherichia coli (E. coli) References: UniProt: P20586, 4-hydroxybenzoate 3-monooxygenase | ||||||
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#2: Chemical | ChemComp-SO4 / #3: Chemical | #4: Chemical | ChemComp-FAD / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.56 Å3/Da / Density % sol: 51.86 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 303 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 100mM potassium phosphate, 0.05 mM glutathion, 30mM sodium sulfite 0.02mM FAD, 450mM ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 30 ℃ | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 Å |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Jun 17, 2001 / Details: mirrors |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→21.64 Å / Num. all: 30046 / Num. obs: 30046 / % possible obs: 96.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 8.4 % / Biso Wilson estimate: 26.3 Å2 / Rmerge(I) obs: 0.096 |
Reflection shell | Resolution: 2→2.13 Å / Rmerge(I) obs: 0.789 / Num. unique all: 4116 / % possible all: 89.7 |
Reflection | *PLUS Lowest resolution: 21.6 Å / % possible obs: 96.5 % / Num. measured all: 261632 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2→21.64 Å / Isotropic thermal model: anisotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 35.2 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2→21.64 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 21.6 Å / σ(F): 0 / % reflection Rfree: 10 % / Rfactor obs: 0.209 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 35.2 Å2 | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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