+Open data
-Basic information
Entry | Database: PDB / ID: 1k0h | ||||||
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Title | Solution structure of bacteriophage lambda gpFII | ||||||
Components | gpFII | ||||||
Keywords | VIRAL PROTEIN / twisted beta-sandwich | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Enterobacteria phage lambda (virus) | ||||||
Method | SOLUTION NMR / distance geometry simulated annealing | ||||||
Authors | Maxwell, K.L. / Yee, A.A. / Arrowsmith, C.H. / Gold, M. / Davidson, A.R. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2002 Title: The solution structure of the bacteriophage lambda head-tail joining protein, gpFII. Authors: Maxwell, K.L. / Yee, A.A. / Arrowsmith, C.H. / Gold, M. / Davidson, A.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1k0h.cif.gz | 351.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1k0h.ent.gz | 288.7 KB | Display | PDB format |
PDBx/mmJSON format | 1k0h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1k0h_validation.pdf.gz | 341.9 KB | Display | wwPDB validaton report |
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Full document | 1k0h_full_validation.pdf.gz | 442.8 KB | Display | |
Data in XML | 1k0h_validation.xml.gz | 34.1 KB | Display | |
Data in CIF | 1k0h_validation.cif.gz | 44.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/k0/1k0h ftp://data.pdbj.org/pub/pdb/validation_reports/k0/1k0h | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12775.037 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacteria phage lambda (virus) / Genus: Lambda-like viruses / Gene: FII / Plasmid: PET21D / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(LAMBDADE3) / References: UniProt: P03714 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy. |
-Sample preparation
Details | Contents: 1.3mM gpFII U-15N, 13C; 10MM PHOSPHATE BUFFER NA, 200MM NACL Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 200mM NaCl / pH: 6.5 / Pressure: ambient / Temperature: 298 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: distance geometry simulated annealing / Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 10 |