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- PDB-1jyk: Catalytic Mechanism of CTP:phosphocholine Cytidylyltransferase fr... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1jyk | ||||||
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Title | Catalytic Mechanism of CTP:phosphocholine Cytidylyltransferase from Streptococcus pneumoniae (LicC) | ||||||
![]() | CTP:phosphocholine Cytidylyltransferase | ||||||
![]() | TRANSFERASE / LicC / 3D Structure / CTP:phosphocholine cytidylyltransferase | ||||||
Function / homology | ![]() biosynthetic process / nucleotidyltransferase activity / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kwak, B.-Y. / Yun, M. / Park, H.-w. | ||||||
![]() | ![]() Title: Structure and mechanism of CTP:phosphocholine cytidylyltransferase (LicC) from Streptococcus pneumoniae. Authors: Kwak, B.Y. / Zhang, Y.M. / Yun, M. / Heath, R.J. / Rock, C.O. / Jackowski, S. / Park, H.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 61.3 KB | Display | ![]() |
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PDB format | ![]() | 47.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 367.3 KB | Display | ![]() |
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Full document | ![]() | 370.7 KB | Display | |
Data in XML | ![]() | 6.4 KB | Display | |
Data in CIF | ![]() | 10.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 29805.346 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.78 Å3/Da / Density % sol: 55.78 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: sodium citrate, ammonium sulfate, tartrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP at 291K | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / Method: unknown | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | |||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Apr 20, 2001 | |||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 1.5→50 Å / Num. obs: 54137 / % possible obs: 99.9 % / Observed criterion σ(I): -3 / Biso Wilson estimate: 20.6 Å2 / Rsym value: 0.048 / Net I/σ(I): 36.8 | |||||||||
Reflection shell | Resolution: 1.5→1.55 Å / Redundancy: 2.79 % / Num. unique all: 10252 / % possible all: 99.6 | |||||||||
Reflection | *PLUS Lowest resolution: 50 Å / Num. measured all: 1187180 / Rmerge(I) obs: 0.048 | |||||||||
Reflection shell | *PLUS % possible obs: 99.7 % |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 1.5→20 Å
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Refine LS restraints |
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Software | *PLUS Name: XTALVIEW / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.5 Å / Lowest resolution: 20 Å / σ(F): 2 / % reflection Rfree: 5 % / Rfactor obs: 0.218 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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