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Yorodumi- PDB-1jxd: SOLUTION STRUCTURE OF REDUCED CU(I) PLASTOCYANIN FROM SYNECHOCYST... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1jxd | ||||||
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Title | SOLUTION STRUCTURE OF REDUCED CU(I) PLASTOCYANIN FROM SYNECHOCYSTIS PCC6803 | ||||||
Components | PLASTOCYANIN | ||||||
Keywords | ELECTRON TRANSPORT / COPPER PROTEIN BETA BARREL ELECTRON TRANSFER | ||||||
Function / homology | Function and homology information plasma membrane-derived thylakoid membrane / electron transfer activity / copper ion binding Similarity search - Function | ||||||
Biological species | Synechocystis sp. PCC 6803 (bacteria) | ||||||
Method | SOLUTION NMR / ENERGY MINIMIZATION | ||||||
Authors | Bertini, I. / Bryant, D.A. / Ciurli, S. / Dikiy, A. / Fernandez, C.O. / Luchinat, C. / Safarov, N. / Vila, A.J. / Zhao, J. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2001 Title: Backbone dynamics of plastocyanin in both oxidation states. Solution structure of the reduced form and comparison with the oxidized state. Authors: Bertini, I. / Bryant, D.A. / Ciurli, S. / Dikiy, A. / Fernandez, C.O. / Luchinat, C. / Safarov, N. / Vila, A.J. / Zhao, J. #1: Journal: J.Am.Chem.Soc. / Year: 2001 Title: THE FIRST SOLUTION STRUCTURE OF A PARAMAGNETIC COPPER(II) PROTEIN: THE CASE OF OXIDIZED PLASTOCYANIN FROM THE CYANOBACTERIUM SYNECHOCYSTIS PCC6803 Authors: Bertini, I. / Ciurli, S. / Dikiy, A. / Fernandez, C.O. / Luchinat, C. / Safarov, N. / Shumilin, S. / Vila, A.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1jxd.cif.gz | 35.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1jxd.ent.gz | 27 KB | Display | PDB format |
PDBx/mmJSON format | 1jxd.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jx/1jxd ftp://data.pdbj.org/pub/pdb/validation_reports/jx/1jxd | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10249.430 Da / Num. of mol.: 1 / Mutation: D98E Source method: isolated from a genetically manipulated source Source: (gene. exp.) Synechocystis sp. PCC 6803 (bacteria) / Strain: PCC6803 / Plasmid: PET3D / Production host: Escherichia coli (E. coli) / Strain (production host): BLR(DE3) / References: UniProt: P21697 |
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#2: Chemical | ChemComp-CU / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 3 MM PLASTOCYANIN U-15N / Solvent system: 50 MM PHOSPHATE BUFFER |
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Sample conditions | Ionic strength: 50 mM PHOSPHATE / pH: 5.2 / Pressure: AMBIENT / Temperature: 295 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: ENERGY MINIMIZATION / Software ordinal: 1 | ||||||||||||||||
NMR ensemble | Conformers submitted total number: 1 |